1sn6: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1sn6.gif|left|200px]] | [[Image:1sn6.gif|left|200px]] | ||
'''NMR solution structure of human Saposin C in SDS micelles''' | {{Structure | ||
|PDB= 1sn6 |SIZE=350|CAPTION= <scene name='initialview01'>1sn6</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= PSAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''NMR solution structure of human Saposin C in SDS micelles''' | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
1SN6 is a [ | 1SN6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SN6 OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of human saposin C in a detergent environment., Hawkins CA, de Alba E, Tjandra N, J Mol Biol. 2005 Mar 11;346(5):1381-92. Epub 2005 Jan 20. PMID:[http:// | Solution structure of human saposin C in a detergent environment., Hawkins CA, de Alba E, Tjandra N, J Mol Biol. 2005 Mar 11;346(5):1381-92. Epub 2005 Jan 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713488 15713488] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 19: | Line 28: | ||
[[Category: Hawkins, C A.]] | [[Category: Hawkins, C A.]] | ||
[[Category: Tjandra, N.]] | [[Category: Tjandra, N.]] | ||
[[Category: 3 disulfide | [[Category: 3 disulfide bridge]] | ||
[[Category: all alpha- | [[Category: all alpha-helice]] | ||
[[Category: alpha-helices connected by | [[Category: alpha-helices connected by turn]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:07:09 2008'' | ||
Revision as of 12:07, 20 March 2008
| |||||||||||||
| 1sn6 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene: | PSAP (Homo sapiens) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
NMR solution structure of human Saposin C in SDS micelles
Overview
Saposin C is a lysosomal, membrane-binding protein that acts as an activator for the hydrolysis of glucosylceramide by the enzyme glucocerebrosidase. We used high-resolution NMR to determine the three-dimensional solution structure of saposin C in the presence of the detergent sodium dodecyl sulfate (SDS). This structure provides the first representation of membrane bound saposin C at the atomic level. In the presence of SDS, the protein adopts an open conformation with an exposed hydrophobic pocket. In contrast, the previously reported NMR structure of saposin C in the absence of SDS is compact and contains a hydrophobic core that is not exposed to the solvent. NMR data indicate that the SDS molecules interact with the hydrophobic pocket. The structure of saposin C in the presence of SDS is very similar to a monomer in the saposin B homodimer structure. Their comparison reveals possible similarity in the type of protein/lipid interaction as well as structural components differentiating their quaternary structures and functional specificity.
Disease
Known diseases associated with this structure: Combined SAP deficiency OMIM:[176801], Gaucher disease, atypical OMIM:[176801], Krabbe disease, atypical OMIM:[176801], Metachromatic leukodystrophy due to SAP-b deficiency OMIM:[176801]
About this Structure
1SN6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of human saposin C in a detergent environment., Hawkins CA, de Alba E, Tjandra N, J Mol Biol. 2005 Mar 11;346(5):1381-92. Epub 2005 Jan 20. PMID:15713488
Page seeded by OCA on Thu Mar 20 14:07:09 2008
