1sqj: Difference between revisions
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[[Image:1sqj.gif|left|200px]] | [[Image:1sqj.gif|left|200px]] | ||
'''Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)''' | {{Structure | ||
|PDB= 1sqj |SIZE=350|CAPTION= <scene name='initialview01'>1sqj</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Oligoxyloglucan_reducing-end-specific_cellobiohydrolase Oligoxyloglucan reducing-end-specific cellobiohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.150 3.2.1.150] | |||
|GENE= | |||
}} | |||
'''Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1SQJ is a [ | 1SQJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geotrichum_sp._m128 Geotrichum sp. m128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQJ OCA]. | ||
==Reference== | ==Reference== | ||
Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase., Yaoi K, Kondo H, Noro N, Suzuki M, Tsuda S, Mitsuishi Y, Structure. 2004 Jul;12(7):1209-17. PMID:[http:// | Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase., Yaoi K, Kondo H, Noro N, Suzuki M, Tsuda S, Mitsuishi Y, Structure. 2004 Jul;12(7):1209-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242597 15242597] | ||
[[Category: Geotrichum sp. m128]] | [[Category: Geotrichum sp. m128]] | ||
[[Category: Oligoxyloglucan reducing-end-specific cellobiohydrolase]] | [[Category: Oligoxyloglucan reducing-end-specific cellobiohydrolase]] | ||
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[[Category: beta-propeller]] | [[Category: beta-propeller]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:08:21 2008'' | ||
Revision as of 12:08, 20 March 2008
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| 1sqj, resolution 2.20Å | |||||||||||||
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| Activity: | Oligoxyloglucan reducing-end-specific cellobiohydrolase, with EC number 3.2.1.150 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)
Overview
Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH; EC 3.2.1.150) is an exoglucanase that recognizes the reducing end of oligoxyloglucan and releases two glucosyl residue segments from the main chain. The X-ray crystal structure of OXG-RCBH determined at 2.2 A resolution reveals a unique feature of this enzyme; OXG-RCBH consists of a tandem repeat of two similar domains, which are both folded into seven-bladed beta-propeller structures. The sequence alignment of the propeller blades, based on the structure, indicates that a weak repeat of the amino acid sequence occurred seven times to construct each domain. There is a cleft that can accommodate the substrate oligosaccharide between the two domains, which is a putative substrate binding subsite. Mutation of either Asp35 or Asp465, located in the putative catalytic center, to Asn resulted in a protein with no detectable catalytic activity, indicating the critical role of these amino acids in catalysis.
About this Structure
1SQJ is a Single protein structure of sequence from Geotrichum sp. m128. Full crystallographic information is available from OCA.
Reference
Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase., Yaoi K, Kondo H, Noro N, Suzuki M, Tsuda S, Mitsuishi Y, Structure. 2004 Jul;12(7):1209-17. PMID:15242597
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