1st8: Difference between revisions
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[[Image:1st8.gif|left|200px]] | [[Image:1st8.gif|left|200px]] | ||
'''Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus''' | {{Structure | ||
|PDB= 1st8 |SIZE=350|CAPTION= <scene name='initialview01'>1st8</scene>, resolution 2.35Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] | |||
|GENE= 1-feh IIa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13427 Cichorium intybus]) | |||
}} | |||
'''Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ST8 is a [ | 1ST8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cichorium_intybus Cichorium intybus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ST8 OCA]. | ||
==Reference== | ==Reference== | ||
X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus., Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A, Plant J. 2005 Feb;41(3):400-11. PMID:[http:// | X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus., Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A, Plant J. 2005 Feb;41(3):400-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659099 15659099] | ||
[[Category: Cichorium intybus]] | [[Category: Cichorium intybus]] | ||
[[Category: Fructan beta-fructosidase]] | [[Category: Fructan beta-fructosidase]] | ||
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[[Category: five fold beta propeller]] | [[Category: five fold beta propeller]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:09:20 2008'' | ||
Revision as of 12:09, 20 March 2008
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| 1st8, resolution 2.35Å | |||||||||||||
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| Ligands: | GOL | ||||||||||||
| Gene: | 1-feh IIa (Cichorium intybus) | ||||||||||||
| Activity: | Fructan beta-fructosidase, with EC number 3.2.1.80 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus
Overview
Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa from Cichorium intybus is described at a resolution of 2.35 A. The structure consists of an N-terminal fivefold beta-propeller domain connected to two C-terminal beta-sheets. The putative active site is located entirely in the beta-propeller domain and is formed by amino acids which are highly conserved within glycosyl hydrolase family 32. The fructan-binding site is thought to be in the cleft formed between the two domains. The 1-FEH IIa structure is compared with the structures of two homologous but functionally different enzymes: a levansucrase from Bacillus subtilis (glycosyl hydrolase family 68) and an invertase from Thermotoga maritima (glycosyl hydrolase family 32).
About this Structure
1ST8 is a Single protein structure of sequence from Cichorium intybus. Full crystallographic information is available from OCA.
Reference
X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus., Verhaest M, Ende WV, Roy KL, De Ranter CJ, Laere AV, Rabijns A, Plant J. 2005 Feb;41(3):400-11. PMID:15659099
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