1t0o: Difference between revisions
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[[Image:1t0o.gif|left|200px]] | [[Image:1t0o.gif|left|200px]] | ||
'''The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose''' | {{Structure | ||
|PDB= 1t0o |SIZE=350|CAPTION= <scene name='initialview01'>1t0o</scene>, resolution 1.96Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] | |||
|GENE= | |||
}} | |||
'''The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1T0O is a [ | 1T0O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0O OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism., Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I, J Mol Biol. 2004 May 28;339(2):413-22. PMID:[http:// | Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism., Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I, J Mol Biol. 2004 May 28;339(2):413-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15136043 15136043] | ||
[[Category: Alpha-galactosidase]] | [[Category: Alpha-galactosidase]] | ||
[[Category: Hypocrea jecorina]] | [[Category: Hypocrea jecorina]] | ||
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[[Category: complex]] | [[Category: complex]] | ||
[[Category: glycoprotein]] | [[Category: glycoprotein]] | ||
[[Category: two | [[Category: two domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:12:06 2008'' | ||
Revision as of 12:12, 20 March 2008
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| 1t0o, resolution 1.96Å | |||||||||||||
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| Ligands: | GAL | ||||||||||||
| Activity: | Alpha-galactosidase, with EC number 3.2.1.22 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
The structure of alpha-galactosidase from Trichoderma reesei complexed with beta-D-galactose
Overview
The crystal structures of alpha-galactosidase from the mesophilic fungus Trichoderma reesei and its complex with the competitive inhibitor, beta-d-galactose, have been determined at 1.54 A and 2.0 A resolution, respectively. The alpha-galactosidase structure was solved by the quick cryo-soaking method using a single Cs derivative. The refined crystallographic model of the alpha-galactosidase consists of two domains, an N-terminal catalytic domain of the (beta/alpha)8 barrel topology and a C-terminal domain which is formed by an antiparallel beta-structure. The protein contains four N-glycosylation sites located in the catalytic domain. Some of the oligosaccharides were found to participate in inter-domain contacts. The galactose molecule binds to the active site pocket located in the center of the barrel of the catalytic domain. Analysis of the alpha-galactosidase- galactose complex reveals the residues of the active site and offers a structural basis for identification of the putative mechanism of the enzymatic reaction. The structure of the alpha-galactosidase closely resembles those of the glycoside hydrolase family 27. The conservation of two catalytic Asp residues, identified for this family, is consistent with a double-displacement reaction mechanism for the alpha-galactosidase. Modeling of possible substrates into the active site reveals specific hydrogen bonds and hydrophobic interactions that could explain peculiarities of the enzyme kinetics.
About this Structure
1T0O is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.
Reference
Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism., Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I, J Mol Biol. 2004 May 28;339(2):413-22. PMID:15136043
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