Sandbox Reserved 718: Difference between revisions

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Let-7 is a key target of Lin28 but there are four additional functions:

First, during retinoic-acid-induced neurogliogenesis, Lin28a overexpression altered the expression of several transcription factors involved in early embryonic cell fate decision before any increase in let-7 level was detected (Balzer ~~et al~~., 2010). Second, impaired glucose tolerance and insulin resistance were observed in muscle-specific Lin28a knockout mice without significant changes in the let-7 level (Zhu et al., 2011). Third, Lin28A interacts with mRNAs and cosediments with polysome in sucrose gradient centrifugation (Balzer and Moss, 2007). Consistently, several studies reported that Lin28A can bind to and enhance translation of certain mRNAs such as Igf2 in differentiating myoblasts and Oct4 in ESCs (Polesskaya ~~et al~~., 2007; Qiu ~~et al~~., 2010; Xu and Huang, 2009; Xu ~~et al~~., 2009).

First, during retinoic-acid-induced neurogliogenesis, Lin28a overexpression altered the expression of several transcription factors involved in early embryonic cell fate decision before any increase in let-7 level was detected. <ref>Balzer, E., Heine, C., Jiang, Q., Lee, V.M., and Moss, E.G. In: ''LIN28 alters cell fate succession and acts independently of the let-7 microRNA during neurogliogenesis in vitro. Development 137, S.891–900, 2010.</ref> Second, impaired glucose tolerance and insulin resistance were observed in muscle-specific Lin28a knockout mice without significant changes in the let-7 level.<ref>Zhu, H., Shyh-Chang, N., Segre , A.V., Shinoda, G., Shah, S.P., Einhorn, W.S., Takeuchi, A., Engreitz, J.M., Hagan, J.P., Kharas, M.G., et al; DIAGRAM Consortium; MAGIC Investigators. In: ''The Lin28/let-7 axis regulates glucose metabolism.'' Cell 147, S.81–94, 2011.</ref> Third, Lin28A interacts with mRNAs and cosediments with polysome in sucrose gradient centrifugation.<ref>Erica Balzer and Eric G. Moss: ''Localization of the Developmental Timing Regulator Lin28 to mRNP Complexes, P-bodies and Stress Granules.'' In: RNA Biology 4:1, S.16-25, January/February/March 2007.</ref> Consistently, several studies reported that Lin28A can bind to and enhance translation of certain mRNAs such as Igf2 in differentiating myoblasts and Oct4 in ESCs.<ref>Polesskaya, A., Cuvellier, S., Naguibneva, I., Duquet, A., Moss, E.G., and Harel-Bellan, A. In: ''Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency.'' Genes Dev. 21, 1125–1138, 2007.</ref><ref>Qiu, C., Ma, Y., Wang, J., Peng, S., and Huang, Y. In: ''Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells.'' Nucleic Acids Res. 38, S.1240–1248, 2010.</ref><ref>Xu, B., and Huang, Y. In: ''Histone H2a mRNA interacts with Lin28 and contains a Lin28-dependent posttranscriptional regulatory element.'' Nucleic Acids Res. 37, S.4256–4263, 2009.</ref><ref>Xu, B., Zhang, K., and Huang, Y. In: ''Lin28 modulates cell growth and associates with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells.'' RNA 15, S.357–361, 2009.</ref>

== '''Structure''' ==

Sandbox Reserved 718: Difference between revisions

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@@ Line 12: / Line 12: @@
 Let-7 is a key target of Lin28 but there are four additional functions:
-First, during retinoic-acid-induced neurogliogenesis, Lin28a overexpression altered the expression of several transcription factors involved in early embryonic cell fate decision before any increase in let-7 level was detected (Balzer et al., 2010). Second, impaired glucose tolerance and insulin resistance were observed in muscle-specific Lin28a knockout mice without significant changes in the let-7 level (Zhu et al., 2011). Third, Lin28A interacts with mRNAs and cosediments with polysome in sucrose gradient centrifugation (Balzer and Moss, 2007). Consistently, several studies reported that Lin28A can bind to and enhance translation of certain mRNAs such as Igf2 in differentiating myoblasts and Oct4 in ESCs (Polesskaya et al., 2007; Qiu et al., 2010; Xu and Huang, 2009; Xu et al., 2009).
+First, during retinoic-acid-induced neurogliogenesis, Lin28a overexpression altered the expression of several transcription factors involved in early embryonic cell fate decision before any increase in let-7 level was detected. <ref>Balzer, E., Heine, C., Jiang, Q., Lee, V.M., and Moss, E.G. In: ''LIN28 alters cell fate succession and acts independently of the let-7 microRNA during neurogliogenesis in vitro. Development 137, S.891–900, 2010.</ref> Second, impaired glucose tolerance and insulin resistance were observed in muscle-specific Lin28a knockout mice without significant changes in the let-7 level.<ref>Zhu, H., Shyh-Chang, N., Segre , A.V., Shinoda, G., Shah, S.P., Einhorn, W.S., Takeuchi, A., Engreitz, J.M., Hagan, J.P., Kharas, M.G., et al; DIAGRAM Consortium; MAGIC Investigators. In: ''The Lin28/let-7 axis regulates glucose metabolism.'' Cell 147, S.81–94, 2011.</ref> Third, Lin28A interacts with mRNAs and cosediments with polysome in sucrose gradient centrifugation.<ref>Erica Balzer and Eric G. Moss: ''Localization of the Developmental Timing Regulator Lin28 to mRNP Complexes, P-bodies and Stress Granules.'' In: RNA Biology 4:1, S.16-25, January/February/March 2007.</ref> Consistently, several studies reported that Lin28A can bind to and enhance translation of certain mRNAs such as Igf2 in differentiating myoblasts and Oct4 in ESCs.<ref>Polesskaya, A., Cuvellier, S., Naguibneva, I., Duquet, A., Moss, E.G., and Harel-Bellan, A. In: ''Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency.'' Genes Dev. 21, 1125–1138, 2007.</ref><ref>Qiu, C., Ma, Y., Wang, J., Peng, S., and Huang, Y. In: ''Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells.'' Nucleic Acids Res. 38, S.1240–1248, 2010.</ref><ref>Xu, B., and Huang, Y. In: ''Histone H2a mRNA interacts with Lin28 and contains a Lin28-dependent posttranscriptional regulatory element.'' Nucleic Acids Res. 37, S.4256–4263, 2009.</ref><ref>Xu, B., Zhang, K., and Huang, Y. In: ''Lin28 modulates cell growth and associates with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells.'' RNA 15, S.357–361, 2009.</ref>
 == '''Structure''' ==