1t6l: Difference between revisions
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'''Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44''' | {{Structure | ||
|PDB= 1t6l |SIZE=350|CAPTION= <scene name='initialview01'>1t6l</scene>, resolution 1.85Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= UL44 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10359 Human herpesvirus 5]) | |||
}} | |||
'''Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1T6L is a [ | 1T6L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_5 Human herpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6L OCA]. | ||
==Reference== | ==Reference== | ||
The cytomegalovirus DNA polymerase subunit UL44 forms a C clamp-shaped dimer., Appleton BA, Loregian A, Filman DJ, Coen DM, Hogle JM, Mol Cell. 2004 Jul 23;15(2):233-44. PMID:[http:// | The cytomegalovirus DNA polymerase subunit UL44 forms a C clamp-shaped dimer., Appleton BA, Loregian A, Filman DJ, Coen DM, Hogle JM, Mol Cell. 2004 Jul 23;15(2):233-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15260974 15260974] | ||
[[Category: Human herpesvirus 5]] | [[Category: Human herpesvirus 5]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: processivity fold]] | [[Category: processivity fold]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:14:29 2008'' | ||
Revision as of 12:14, 20 March 2008
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| 1t6l, resolution 1.85Å | |||||||||||||
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| Gene: | UL44 (Human herpesvirus 5) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44
Overview
The human cytomegalovirus DNA polymerase consists of a catalytic subunit, UL54, and a presumed processivity factor, UL44. We have solved the crystal structure of residues 1-290 of UL44 to 1.85 A resolution by multiwavelength anomalous dispersion. The structure reveals a dimer of UL44 in the shape of a C clamp. Each monomer of UL44 shares its overall fold with other processivity factors, including herpes simplex virus UL42, which is a monomer that binds DNA directly, and the sliding clamp, PCNA, which is a trimer that surrounds DNA, although these proteins share no obvious sequence homology. Analytical ultracentrifugation and gel filtration measurements demonstrated that UL44 also forms a dimer in solution, and substitution of large hydrophobic residues along the homodimer interface with alanine disrupted dimerization and decreased DNA binding. UL44 represents a hybrid processivity factor as it binds DNA directly like UL42, but forms a C clamp that may surround DNA like PCNA.
About this Structure
1T6L is a Single protein structure of sequence from Human herpesvirus 5. Full crystallographic information is available from OCA.
Reference
The cytomegalovirus DNA polymerase subunit UL44 forms a C clamp-shaped dimer., Appleton BA, Loregian A, Filman DJ, Coen DM, Hogle JM, Mol Cell. 2004 Jul 23;15(2):233-44. PMID:15260974
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