1t7d: Difference between revisions
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'''Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor''' | {{Structure | ||
|PDB= 1t7d |SIZE=350|CAPTION= <scene name='initialview01'>1t7d</scene>, resolution 2.47Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ARY:ARYLOMYCIN A2'>ARY</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Signal_peptidase_I Signal peptidase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.89 3.4.21.89] | |||
|GENE= LEPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1T7D is a [ | 1T7D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7D OCA]. | ||
==Reference== | ==Reference== | ||
Crystallographic and biophysical analysis of a bacterial signal peptidase in complex with a lipopeptide-based inhibitor., Paetzel M, Goodall JJ, Kania M, Dalbey RE, Page MG, J Biol Chem. 2004 Jul 16;279(29):30781-90. Epub 2004 May 10. PMID:[http:// | Crystallographic and biophysical analysis of a bacterial signal peptidase in complex with a lipopeptide-based inhibitor., Paetzel M, Goodall JJ, Kania M, Dalbey RE, Page MG, J Biol Chem. 2004 Jul 16;279(29):30781-90. Epub 2004 May 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15136583 15136583] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Signal peptidase I]] | [[Category: Signal peptidase I]] | ||
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[[Category: signal peptide]] | [[Category: signal peptide]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:14:46 2008'' | ||
Revision as of 12:14, 20 March 2008
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| 1t7d, resolution 2.47Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | ARY | ||||||||||||
| Gene: | LEPB (Escherichia coli) | ||||||||||||
| Activity: | Signal peptidase I, with EC number 3.4.21.89 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of Escherichia coli type I signal peptidase in complex with a lipopeptide inhibitor
Overview
We report here the crystallographic and biophysical analysis of a soluble, catalytically active fragment of the Escherichia coli type I signal peptidase (SPase Delta2-75) in complex with arylomycin A2. The 2.5-A resolution structure revealed that the inhibitor is positioned with its COOH-terminal carboxylate oxygen (O45) within hydrogen bonding distance of all the functional groups in the catalytic center of the enzyme (Ser90 O-gamma, Lys145 N-zeta, and Ser88 O-gamma) and that it makes beta-sheet type interactions with the beta-strands that line each side of the binding site. Ligand binding studies, calorimetry, fluorescence spectroscopy, and stopped-flow kinetics were also used to analyze the binding mode of this unique non-covalently bound inhibitor. The crystal structure was solved in the space group P4(3)2(1)2. A detailed comparison is made to the previously published acyl-enzyme inhibitor complex structure (space group: P2(1)2(1)2) and the apo-enzyme structure (space group: P4(1)2(1)2). Together this work provides insights into the binding of pre-protein substrates to signal peptidase and will prove helpful in the development of novel antibiotics.
About this Structure
1T7D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystallographic and biophysical analysis of a bacterial signal peptidase in complex with a lipopeptide-based inhibitor., Paetzel M, Goodall JJ, Kania M, Dalbey RE, Page MG, J Biol Chem. 2004 Jul 16;279(29):30781-90. Epub 2004 May 10. PMID:15136583
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Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Escherichia coli
- Signal peptidase I
- Single protein
- Dalbey, R E.
- Goodall, J J.
- Kania, M.
- Paetzel, M.
- Page, M G.P.
- ARY
- Antibiotic
- Leader peptidase
- Leader peptide
- Lysine general base
- Non-covalently bound inhibitor
- Non-ribosomal peptide synthesis
- Peptide
- Secondary metabolite
- Ser/lys dyad
- Signal peptidase
- Signal peptide
