1t8z: Difference between revisions
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'''Atomic Structure of A Novel Tryptophan-Zipper Pentamer''' | {{Structure | ||
|PDB= 1t8z |SIZE=350|CAPTION= <scene name='initialview01'>1t8z</scene>, resolution 1.45Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=12P:DODECAETHYLENE GLYCOL'>12P</scene> | |||
|ACTIVITY= | |||
|GENE= LPP, MLPA, MULI, B1677, C2072, Z2705, ECS2384, SF1706, S1839 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Atomic Structure of A Novel Tryptophan-Zipper Pentamer''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1T8Z is a [ | 1T8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8Z OCA]. | ||
==Reference== | ==Reference== | ||
Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:[http:// | Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15520380 15520380] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tryptophan-zipper]] | [[Category: tryptophan-zipper]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:15:25 2008'' | ||
Revision as of 12:15, 20 March 2008
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| 1t8z, resolution 1.45Å | |||||||||||||
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| Ligands: | SO4 and 12P | ||||||||||||
| Gene: | LPP, MLPA, MULI, B1677, C2072, Z2705, ECS2384, SF1706, S1839 (Escherichia coli) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Atomic Structure of A Novel Tryptophan-Zipper Pentamer
Overview
Coiled-coil motifs are ubiquitous mediators of specific protein-protein interactions through the formation of interlocking hydrophobic seams between alpha-helical chains. Residues that form these seams occur at the first (a) and fourth (d) positions of a characteristic 7-aa repeat and are primarily aliphatic. The potential of aromatic residues to promote helix association in a coiled coil was explored by engineering a "Trp-zipper" protein with Trp residues at all 14 a and d positions. The protein forms a discrete, stable, alpha-helical pentamer in water at physiological pH. Its 1.45-A crystal structure reveals a parallel, five-stranded coiled coil, a previously uncharacterized type of "knobs-into-holes" packing interaction between interfacial Trp side chains, and an unusual approximately 8-A-diameter axial channel lined with indole rings that is filled with polyethylene glycol 400 and water and sulfate ion molecules. The engineered Trp-zipper pentamer enlarges current views of coiled-coil assembly, molecular recognition, and protein engineering, and may serve as a soluble model for membrane ion channels.
About this Structure
1T8Z is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Atomic structure of a tryptophan-zipper pentamer., Liu J, Yong W, Deng Y, Kallenbach NR, Lu M, Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16156-61. Epub 2004 Nov 1. PMID:15520380
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