1ecy: Difference between revisions
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==Overview== | ==Overview== | ||
Ecotin, a homodimeric protein composed of 142 residue subunits, is a novel, serine protease inhibitor present in Escherichia coli. Its thermostability, and acid stability, as well as broad specificity toward proteases, make it, an interesting protein for structural characterization. Its structure in, the uncomplexed state, determined for two different crystalline, environments, allows a structural comparison of the free inhibitor with, that in complex with trypsin. Although there is no gross structural, rearrangement of ecotin when binding trypsin, the loops involved in, binding trypsin show relatively large shifts in atomic positions. The, inherent flexibility of the loops and the highly nonglobular shape are the, two features essential for its inhibitory function. An insight into the, . | Ecotin, a homodimeric protein composed of 142 residue subunits, is a novel, serine protease inhibitor present in Escherichia coli. Its thermostability, and acid stability, as well as broad specificity toward proteases, make it, an interesting protein for structural characterization. Its structure in, the uncomplexed state, determined for two different crystalline, environments, allows a structural comparison of the free inhibitor with, that in complex with trypsin. Although there is no gross structural, rearrangement of ecotin when binding trypsin, the loops involved in, binding trypsin show relatively large shifts in atomic positions. The, inherent flexibility of the loops and the highly nonglobular shape are the, two features essential for its inhibitory function. An insight into the, understanding of the structural basis of thermostability and acid, stability of ecotin is also provided by the present structure. | ||
==About this Structure== | ==About this Structure== | ||
1ECY is a | 1ECY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GLC as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: P1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ECY OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: serine protease inhibitor]] | [[Category: serine protease inhibitor]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:40:48 2007'' | ||
