1t98: Difference between revisions
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[[Image:1t98.gif|left|200px]] | [[Image:1t98.gif|left|200px]] | ||
'''Crystal Structure of MukF(1-287)''' | {{Structure | ||
|PDB= 1t98 |SIZE=350|CAPTION= <scene name='initialview01'>1t98</scene>, resolution 2.90Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= MUKF, KICB, B0922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Crystal Structure of MukF(1-287)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1T98 is a [ | 1T98 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. | ||
==Reference== | ==Reference== | ||
The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins., Fennell-Fezzie R, Gradia SD, Akey D, Berger JM, EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:[http:// | The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins., Fennell-Fezzie R, Gradia SD, Akey D, Berger JM, EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15902272 15902272] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: winged helix]] | [[Category: winged helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:15:32 2008'' | ||
Revision as of 12:15, 20 March 2008
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| 1t98, resolution 2.90Å | |||||||||||||
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| Gene: | MUKF, KICB, B0922 (Escherichia coli) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of MukF(1-287)
Overview
The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly.
About this Structure
1T98 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins., Fennell-Fezzie R, Gradia SD, Akey D, Berger JM, EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:15902272
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