1qbp: Difference between revisions

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[[~~Image~~:1qbp.~~png|left|200px~~]]

==CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS==

== Structural highlights ==

<table><tr><td colspan='2'>[[1qbp]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QBP FirstGlance]. <br>

</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBV:5-BROMOCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>CBV</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qbp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qbp RCSB], [http://www.ebi.ac.uk/pdbsum/1qbp PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The X-ray crystal structure of a brominated RNA helix with four mismatched base pairs and sequence r(UG(Br)C(Br)CAGUUCGCUGGC)(2) was determined to 2.1 A using the methods of multiwavelength anomalous diffraction (MAD) applied to the bromine K-absorption edge. There are three molecules in the asymmetric unit with unique crystal-packing environments, revealing true conformational variability at high resolution for this sequence. The structure shows that the sequence itself does not define a consistent pattern of solvent molecules, with the exception of the mismatched base pairs, implying that specific RNA-protein interactions would occur only with the nucleotides. There are a number of significant tertiary interactions, some of which are a result of the brominated base pairs and others that are directly mediated by the RNA 2' hydroxyl groups. The mismatched base pairs exhibit a solvent network as well as a stacking pattern with their nearest neighbors that validate previous thermodynamic analysis.

~~{{STRUCTURE_1qbp| PDB=1qbp | SCENE= }}~~

Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability.,Anderson AC, O'Neil RH, Filman DJ, Frederick CA Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:10504226<ref>PMID:10504226</ref>

~~===CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_10504226}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[1qbp]] is a 6 chain structure. Full crystallographic information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1QBP OCA].~~

</StructureSection>

[[Category: Anderson, A C.]]

[[Category: Filman, D J.]]

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@@ Line 1: / Line 1: @@
-[[Image:1qbp.png|left|200px]]
+==CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS==
+<StructureSection load='1qbp' size='340' side='right' caption='[[1qbp]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1qbp]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QBP FirstGlance]. <br>
+</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBV:5-BROMOCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>CBV</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qbp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qbp RCSB], [http://www.ebi.ac.uk/pdbsum/1qbp PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The X-ray crystal structure of a brominated RNA helix with four mismatched base pairs and sequence r(UG(Br)C(Br)CAGUUCGCUGGC)(2) was determined to 2.1 A using the methods of multiwavelength anomalous diffraction (MAD) applied to the bromine K-absorption edge. There are three molecules in the asymmetric unit with unique crystal-packing environments, revealing true conformational variability at high resolution for this sequence. The structure shows that the sequence itself does not define a consistent pattern of solvent molecules, with the exception of the mismatched base pairs, implying that specific RNA-protein interactions would occur only with the nucleotides. There are a number of significant tertiary interactions, some of which are a result of the brominated base pairs and others that are directly mediated by the RNA 2' hydroxyl groups. The mismatched base pairs exhibit a solvent network as well as a stacking pattern with their nearest neighbors that validate previous thermodynamic analysis.
-{{STRUCTURE_1qbp|  PDB=1qbp  |  SCENE=  }}
+Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability.,Anderson AC, O'Neil RH, Filman DJ, Frederick CA Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:10504226<ref>PMID:10504226</ref>
-===CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_10504226}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1qbp]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBP OCA].
+</StructureSection>
 [[Category: Anderson, A C.]]
 [[Category: Filman, D J.]]