Sandbox Reserved 704: Difference between revisions

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2) CP (Connective Peptide)-core and 3) CP1 hairpin consist of antiparallel β-sheets, but only the CP core is fixed on the aminoacylation domain. There is a “joint” formed by the junction of the CP-core and the CP1 hairpin (Ala180 and His462 residues) which is essential for accommodating the tRNA <ref name="">PMID:16155584</ref>.

4) CP1 editing domain is located in the middle of the CP1 hairpin. It is the editing domain, that means that this region is responsible for the hydrolysis of the incorrectly synthesized products. Because LeuRS have a specific editing activity that hydrolyzes misactivated aminoacyl-adenylates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing) or both when they have misformed with noncognate amino acids.The residue Asp 332 is necessary for post-transfer editing <ref name="Fukunaga">PMID:15663927</ref>.

4) <scene name='Sandbox_Reserved_704/Domain_4/1'>CP1 editing domain</scene> is located in the middle of the CP1 hairpin. It is the editing domain, that means that this region is responsible for the hydrolysis of the incorrectly synthesized products. Because LeuRS have a specific editing activity that hydrolyzes misactivated aminoacyl-adenylates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing) or both when they have misformed with noncognate amino acids.The residue Asp 332 is necessary for post-transfer editing <ref name="Fukunaga">PMID:15663927</ref>.

5) CP2 is inserted between the second and third β-sheets of the CP core. It is composed of a pair of antiparallel α-helices and a connecting β-sheet and is necessary for the amino acid activation and post-transfer editing.

Sandbox Reserved 704: Difference between revisions

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@@ Line 25: / Line 25: @@
 ) CP (Connective Peptide)-core and 3) CP1 hairpin consist of antiparallel β-sheets, but only the CP core is fixed on the aminoacylation domain. There is a “joint” formed by the junction of the CP-core and the CP1 hairpin (Ala180 and His462 residues) which is essential for accommodating the tRNA <ref name="">PMID:16155584</ref>.
-) CP1 editing domain is located in the middle of the CP1 hairpin. It is the editing domain, that means that this region is responsible for the hydrolysis of the incorrectly synthesized products. Because LeuRS have a specific editing activity that hydrolyzes misactivated aminoacyl-adenylates  (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing) or both when they have misformed with noncognate amino acids.The residue Asp 332 is necessary for post-transfer editing <ref name="Fukunaga">PMID:15663927</ref>.
+) <scene name='Sandbox_Reserved_704/Domain_4/1'>CP1 editing domain</scene> is located in the middle of the CP1 hairpin. It is the editing domain, that means that this region is responsible for the hydrolysis of the incorrectly synthesized products. Because LeuRS have a specific editing activity that hydrolyzes misactivated aminoacyl-adenylates  (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing) or both when they have misformed with noncognate amino acids.The residue Asp 332 is necessary for post-transfer editing <ref name="Fukunaga">PMID:15663927</ref>.
 ) CP2 is inserted between the second and third β-sheets of the CP core. It is composed of a pair of antiparallel α-helices and a connecting β-sheet and is necessary for the amino acid activation and post-transfer editing.