Sandbox Reserved 717: Difference between revisions

Line 44:

==='''The 33kDa Fragment''' <ref name=r3>PMID: 12888344</ref> <ref>PMID:11530935</ref> ===

The 33kDa fragment (PDB:1H6W) was generated in the presence of EDTA <ref name=r3>PMID: 12888344</ref>. This fragment contains the residues <scene name='Sandbox_Reserved_717/Myfirstscene/1'>85-395</scene> and <scene name='Sandbox_Reserved_717/Mysecondscene/1'>518-527</scene> . The residues 397-517 are lacking because of internal deletion.

The <scene name='Sandbox_Reserved_717/Myscene/11'>33kDa fragment</scene> (PDB:1H6W) was generated in the presence of EDTA <ref name=r3>PMID: 12888344</ref>. This fragment contains the residues <scene name='Sandbox_Reserved_717/Myfirstscene/1'>85-395</scene> and <scene name='Sandbox_Reserved_717/Mysecondscene/1'>518-527</scene> . The residues 397-517 are lacking because of internal deletion.

The 33kDa fragment can be further sub-divided into two subunits <ref name=r3>PMID: 12888344</ref>. The <scene name='Sandbox_Reserved_717/Mythirdscene/1'>neck</scene> (residue 333-341) and the collar (residues <scene name='Sandbox_Reserved_717/Mythirdscene/2'>342-396 </scene> plus <scene name='Sandbox_Reserved_717/Mythirdscene/3'> 518-527</scene>). The neck connects the body of the fibre to its C-terminal collar and receptor-binding site. It consists of a triple alpha-helix which is built by the residues 333-341.

The 33kDa fragment can be further sub-divided into two subunits <ref name=r3>PMID: 12888344</ref>. The <scene name='Sandbox_Reserved_717/Mythirdscene/1'>neck</scene> (residue 333-341) and the <scene name='Sandbox_Reserved_717/Myscene/12'>collar</scene> (residues <scene name='Sandbox_Reserved_717/Mythirdscene/2'>342-396 </scene> plus <scene name='Sandbox_Reserved_717/Mythirdscene/3'> 518-527</scene>). The neck connects the body of the fibre to its C-terminal collar and receptor-binding site. It consists of a triple alpha-helix which is built by the residues 333-341.

The collar domain is a small globular domain. It contains six beta-strands and an alpha-helix <ref name=r3>PMID: 12888344</ref>.

==='''The 45kDa Fragment <ref name=r3>PMID: 12888344</ref> ===

For generating the 45kDa fragment the full length gp12 was co-expressed with its chaperone gp57 and purified <ref name=r3>PMID: 12888344</ref>. Like the 33kDa fragment it also starts with the amino acid Leu85. The 45kDa fragment contains the residues <scene name='Sandbox_Reserved_717/Mythirdscene/4'>397-517</scene> , which are in the 33kDa fragment internal deleted <ref name=r3>PMID: 12888344</ref> .

Like the 33kDa fragment the 45kDa fragment can also be divided into two subunits. These two subunits are called head (residues <scene name='Sandbox_Reserved_717/Mythirdscene/5'>397-446 </scene> and residues <scene name='Sandbox_Reserved_717/Mythirdscene/6'>487-517</scene> ) and <scene name='Sandbox_Reserved_717/Mythirdscene/7'>bonnet</scene> (residues 447-487). On the border between the head and the bonnet subunit there is a metal-binding site <ref name=r3>PMID: 12888344</ref>.

Like the 33kDa fragment the 45kDa fragment can also be divided into two subunits. These two subunits are called <scene name='Sandbox_Reserved_717/Myscene/13'>head </scene> (residues <scene name='Sandbox_Reserved_717/Mythirdscene/5'>397-446 </scene> and residues <scene name='Sandbox_Reserved_717/Mythirdscene/6'>487-517</scene> ) and <scene name='Sandbox_Reserved_717/Mythirdscene/7'>bonnet</scene> (residues 447-487). On the border between the head and the bonnet subunit there is a metal-binding site <ref name=r3>PMID: 12888344</ref>.

==='''Receptor-Binding Domain '''<ref name=r3>PMID: 12888344</ref> ===

Sandbox Reserved 717: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 44: / Line 44: @@
 ==='''The 33kDa Fragment''' <ref name=r3>PMID: 12888344</ref> <ref>PMID:11530935</ref> ===
-The 33kDa fragment (PDB:1H6W)  was generated in the presence of EDTA <ref name=r3>PMID: 12888344</ref>. This fragment contains the residues <scene name='Sandbox_Reserved_717/Myfirstscene/1'>85-395</scene> and <scene name='Sandbox_Reserved_717/Mysecondscene/1'>518-527</scene> .  The residues 397-517 are lacking because of internal deletion.
+The <scene name='Sandbox_Reserved_717/Myscene/11'>33kDa fragment</scene> (PDB:1H6W)  was generated in the presence of EDTA <ref name=r3>PMID: 12888344</ref>. This fragment contains the residues <scene name='Sandbox_Reserved_717/Myfirstscene/1'>85-395</scene> and <scene name='Sandbox_Reserved_717/Mysecondscene/1'>518-527</scene> .  The residues 397-517 are lacking because of internal deletion.
-The 33kDa fragment can be further sub-divided into two subunits <ref name=r3>PMID: 12888344</ref>. The <scene name='Sandbox_Reserved_717/Mythirdscene/1'>neck</scene>  (residue 333-341) and the collar (residues <scene name='Sandbox_Reserved_717/Mythirdscene/2'>342-396 </scene> plus <scene name='Sandbox_Reserved_717/Mythirdscene/3'> 518-527</scene>). The neck connects the body of the fibre to its C-terminal collar and receptor-binding site. It consists of a triple alpha-helix which is built by the residues 333-341.
+The 33kDa fragment can be further sub-divided into two subunits <ref name=r3>PMID: 12888344</ref>. The <scene name='Sandbox_Reserved_717/Mythirdscene/1'>neck</scene>  (residue 333-341) and the <scene name='Sandbox_Reserved_717/Myscene/12'>collar</scene> (residues <scene name='Sandbox_Reserved_717/Mythirdscene/2'>342-396 </scene> plus <scene name='Sandbox_Reserved_717/Mythirdscene/3'> 518-527</scene>). The neck connects the body of the fibre to its C-terminal collar and receptor-binding site. It consists of a triple alpha-helix which is built by the residues 333-341.
 The collar domain is a small globular domain. It contains six beta-strands and an alpha-helix <ref name=r3>PMID: 12888344</ref>.
 ==='''The 45kDa Fragment <ref name=r3>PMID: 12888344</ref> ===
 For generating the 45kDa fragment the full length gp12 was co-expressed with its chaperone gp57 and purified <ref name=r3>PMID: 12888344</ref>. Like the 33kDa fragment it also starts with the amino acid Leu85. The 45kDa fragment contains the residues <scene name='Sandbox_Reserved_717/Mythirdscene/4'>397-517</scene> , which are in the 33kDa fragment internal deleted <ref name=r3>PMID: 12888344</ref> .
-Like the 33kDa fragment the 45kDa fragment can also be divided into two subunits. These two subunits are called head (residues <scene name='Sandbox_Reserved_717/Mythirdscene/5'>397-446 </scene> and residues <scene name='Sandbox_Reserved_717/Mythirdscene/6'>487-517</scene> ) and <scene name='Sandbox_Reserved_717/Mythirdscene/7'>bonnet</scene>  (residues 447-487). On the border between the head and the bonnet subunit there is a metal-binding site <ref name=r3>PMID: 12888344</ref>.
+Like the 33kDa fragment the 45kDa fragment can also be divided into two subunits. These two subunits are called <scene name='Sandbox_Reserved_717/Myscene/13'>head </scene> (residues <scene name='Sandbox_Reserved_717/Mythirdscene/5'>397-446 </scene> and residues <scene name='Sandbox_Reserved_717/Mythirdscene/6'>487-517</scene> ) and <scene name='Sandbox_Reserved_717/Mythirdscene/7'>bonnet</scene>  (residues 447-487). On the border between the head and the bonnet subunit there is a metal-binding site <ref name=r3>PMID: 12888344</ref>.
 ==='''Receptor-Binding Domain '''<ref name=r3>PMID: 12888344</ref> ===