Sandbox Reserved 704: Difference between revisions

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Other the Rossmann fold domain, there are other fused and inserted domains:

2) The <scene name='Sandbox_Reserved_704/Domain_2/1'>CP (Connective Peptide)-core</scene> and 3) The <scene name='Sandbox_Reserved_704/Domain_3/2'>CP1 hairpin</scene> consist of antiparallel β-sheets, but only the CP-core is fixed on the aminoacylation domain. There is a “joint” formed by the junction of the CP-core and the CP1 hairpin (Ala180 and His462 residues) which is essential for accommodating the tRNA <ref name="">PMID:16155584</ref>.

2) The <scene name='Sandbox_Reserved_704/Domain_2/1'>CP (Connective Peptide)-core</scene> and 3) The <scene name='Sandbox_Reserved_704/Domain_3/2'>CP1 hairpin</scene> consist of antiparallel β-sheets, but only the CP-core is fixed on the aminoacylation domain. There is a <scene name='Sandbox_Reserved_704/Joint/1'>“joint”</scene> formed by the junction of the CP-core and the CP1 hairpin (Ala180 and His462 residues) which is essential for accommodating the tRNA <ref name="">PMID:16155584</ref>.

4) The <scene name='Sandbox_Reserved_704/Domain_4/1'>CP1 editing domain</scene> is located in the middle of the CP1 hairpin. It is the editing domain, that means that this region is responsible for the hydrolysis of the incorrectly synthesized products. Because LeuRS have a specific editing activity that hydrolyzes misactivated aminoacyl-adenylates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing) or both when they have misformed with noncognate amino acids.The residue Asp 332 is necessary for post-transfer editing <ref name="Fukunaga">PMID:15663927</ref>.

Sandbox Reserved 704: Difference between revisions

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@@ Line 23: / Line 23: @@
 Other the Rossmann fold domain, there are other fused and inserted domains:
-) The <scene name='Sandbox_Reserved_704/Domain_2/1'>CP (Connective Peptide)-core</scene> and 3) The <scene name='Sandbox_Reserved_704/Domain_3/2'>CP1 hairpin</scene> consist of antiparallel β-sheets, but only the CP-core is fixed on the aminoacylation domain. There is a “joint” formed by the junction of the CP-core and the CP1 hairpin (Ala180 and His462 residues) which is essential for accommodating the tRNA <ref name="">PMID:16155584</ref>.
+) The <scene name='Sandbox_Reserved_704/Domain_2/1'>CP (Connective Peptide)-core</scene> and 3) The <scene name='Sandbox_Reserved_704/Domain_3/2'>CP1 hairpin</scene> consist of antiparallel β-sheets, but only the CP-core is fixed on the aminoacylation domain. There is a <scene name='Sandbox_Reserved_704/Joint/1'>“joint”</scene> formed by the junction of the CP-core and the CP1 hairpin (Ala180 and His462 residues) which is essential for accommodating the tRNA <ref name="">PMID:16155584</ref>.
 ) The <scene name='Sandbox_Reserved_704/Domain_4/1'>CP1 editing domain</scene> is located in the middle of the CP1 hairpin. It is the editing domain, that means that this region is responsible for the hydrolysis of the incorrectly synthesized products. Because LeuRS have a specific editing activity that hydrolyzes misactivated aminoacyl-adenylates  (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing) or both when they have misformed with noncognate amino acids.The residue Asp 332 is necessary for post-transfer editing <ref name="Fukunaga">PMID:15663927</ref>.