Sandbox Reserved 718: Difference between revisions
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== '''Structure''' == | == '''Structure''' == | ||
Lin28 consists of two domains: a cold shock domain and a <scene name='Sandbox_Reserved_718/Zfd/1'>pair of CCHC zinc fingers</scene>. | Lin28 consists of two domains: a cold shock domain and a <scene name='Sandbox_Reserved_718/Zfd/1'>pair of CCHC zinc fingers</scene>. | ||
Both are RNA binding domains. The cold-shock domain (CSD) at the N-terminal part of lin28A interacts with a loop, with a GNGAY motif, of the RNA. | Both are RNA binding domains. The cold-shock domain (CSD) at the N-terminal part of lin28A interacts with a loop, with a GNGAY motif, of the RNA. | ||
The other domain has the binding element AAGNNG (most frequent sequence: AAGGAG) which can be found downstream of the GNGAY motif. The first G builds a <scene name='Sandbox_Reserved_718/Lin28a_let-7/2'>hydrogen bond</scene> with <scene name='Sandbox_Reserved_718/Lys160/2'>LYS160</scene> in the zinc finger motif. <ref>Jun Cho, Hyeshik Chang, S. Chul Kwon, Baekgyu Kim, Yoosik Kim, Junho Choe, Minju Ha, Yoon Ki Kim, and V. Narry Kim: ''Lin28A Is a Suppressor of ER-Associated Translation in Embryonic Stem Cells.'' In: Cell 151, S.765–777, November 9, 2012</ref> | The other domain has the binding element AAGNNG (most frequent sequence: AAGGAG) which can be found downstream of the GNGAY motif. <Structure load='2li8' size='300' frame='true' align='right' caption='pdb 2li8 crystall structure lin28A/let-7' scene='Sandbox_Reserved_718/Lin28a_let-7/1' />The first G builds a <scene name='Sandbox_Reserved_718/Lin28a_let-7/2'>hydrogen bond</scene> with <scene name='Sandbox_Reserved_718/Lys160/2'>LYS160</scene> in the zinc finger motif. <ref>Jun Cho, Hyeshik Chang, S. Chul Kwon, Baekgyu Kim, Yoosik Kim, Junho Choe, Minju Ha, Yoon Ki Kim, and V. Narry Kim: ''Lin28A Is a Suppressor of ER-Associated Translation in Embryonic Stem Cells.'' In: Cell 151, S.765–777, November 9, 2012</ref> | ||
When lin28A interacts with <scene name='Sandbox_Reserved_718/Let-7/1'>let-7</scene>(main target), they build a complex with a crystallographic structure together. | When lin28A interacts with <scene name='Sandbox_Reserved_718/Let-7/1'>let-7</scene>(main target), they build a complex with a crystallographic structure together. | ||
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Katrin Frohnmüller, Teresa Wiese | Katrin Frohnmüller, Teresa Wiese | ||
