TAL effector: Difference between revisions

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<br>[[Image:nihms396484f3.jpg|left|400px|thumb|Fig.3]]<br>

'''Figure 3''': All of the repeats in the DNA-bound PthXo1 structure form highly similar two-helix bundles (Figure 1c). The helices span positions 3 to 11 and 14 to 33, locating the RVD in a loop between them. A proline located at position 27, creates a kink in the second helix that appears to be critical for the sequential packing and association of tandem repeats with the DNA double helix. The packing of consecutive helices within and between individual repeats is left-handed, in contrast to the right-handed packing of helices found in TPR proteins (10). The modular architecture of the TAL effector repeats is reminiscent of the mitochondrial transcription terminator [http://www.proteopedia.org/wiki/index.php/3n6s mTERF] (11) and the [http://www.proteopedia.org/wiki/index.php/TRAP RNA-binding attenuation protein TRAP] (12);

'''Figure 3''': All of the repeats in the DNA-bound PthXo1 structure form highly similar two-helix bundles (Figure 1c). The helices span positions 3 to 11 and 14 to 33, locating the RVD in a loop between them. A proline located at position 27, creates a kink in the second helix that appears to be critical for the sequential packing and association of tandem repeats with the DNA double helix. The packing of consecutive helices within and between individual repeats is left-handed, in contrast to the right-handed packing of helices found in TPR proteins (10). The modular architecture of the TAL effector repeats is reminiscent of the mitochondrial transcription terminator [http://www.proteopedia.org/wiki/index.php/3n6s mTERF] (11) and the [http://www.proteopedia.org/wiki/index.php/TRAP RNA-binding attenuation protein TRAP] (12).

Sequence-specific contacts of PthXo1 to the DNA are made exclusively by the second residue in each RVD to the corresponding base on the sense strand. In contrast, the side chain at the first position of each RVD contacts the backbone carbonyl oxygen of position 8 in each repeat, constraining the RVD-containing loop (Figure 3). Additional, nonspecific contacts to the DNA are made by a lysine and glutamine found at positions 16 and 17.

==Engineering==

TAL effector: Difference between revisions

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@@ Line 47: / Line 47: @@
 <br>[[Image:nihms396484f3.jpg|left|400px|thumb|Fig.3]]<br>
-'''Figure 3''': All of the repeats in the DNA-bound PthXo1 structure form highly similar two-helix bundles (Figure 1c). The helices span positions 3 to 11 and 14 to 33, locating the RVD in a loop between them. A proline located at position 27, creates a kink in the second helix that appears to be critical for the sequential packing and association of tandem repeats with the DNA double helix. The packing of consecutive helices within and between individual repeats is left-handed, in contrast to the right-handed packing of helices found in TPR proteins (10). The modular architecture of the TAL effector repeats is reminiscent of the mitochondrial transcription terminator [http://www.proteopedia.org/wiki/index.php/3n6s mTERF] (11) and the [http://www.proteopedia.org/wiki/index.php/TRAP RNA-binding attenuation protein TRAP] (12);
+'''Figure 3''': All of the repeats in the DNA-bound PthXo1 structure form highly similar two-helix bundles (Figure 1c). The helices span positions 3 to 11 and 14 to 33, locating the RVD in a loop between them. A proline located at position 27, creates a kink in the second helix that appears to be critical for the sequential packing and association of tandem repeats with the DNA double helix. The packing of consecutive helices within and between individual repeats is left-handed, in contrast to the right-handed packing of helices found in TPR proteins (10). The modular architecture of the TAL effector repeats is reminiscent of the mitochondrial transcription terminator [http://www.proteopedia.org/wiki/index.php/3n6s mTERF] (11) and the [http://www.proteopedia.org/wiki/index.php/TRAP RNA-binding attenuation protein TRAP] (12).
+Sequence-specific contacts of PthXo1 to the DNA are made exclusively by the second residue in each RVD to the corresponding base on the sense strand. In contrast, the side chain at the first position of each RVD contacts the backbone carbonyl oxygen of position 8 in each repeat, constraining the RVD-containing loop (Figure 3). Additional, nonspecific contacts to the DNA are made by a lysine and glutamine found at positions 16 and 17.
 ==Engineering==