Sandbox Reserved 709: Difference between revisions

Line 52:

Here, as the structure begins at the amino acid number 70, we cannot see the IBB which is located in the 2-60 region.

* '''A 50kDa C-terminal''' <scene name='Sandbox_Reserved_709/Importin_nlsbs/1'>NLS binding sites</scene> contained in <scene name='Sandbox_Reserved_709/Importin_arm/3'>10 tandem armadillo (Arm) repeats</scene>. These arm repeat domains have an elongated superhelical structure and each of them contains 3 α-helices (H1, H2, H3). Together, H3 helices define the inner concave surface of the protein and the NLS-binding ~~sites. Indeed, in fact there are two NLS-binding sites : a major one and a minor one (determined by their affinity for NLS)~~. <ref>PMID:22248489</ref> The main chain of the cNLs runs antiparallel to the direction of the importin α superhelix, with the cNLS backbone interacting with an array of conserved asparagine residues in consecutive Arm repeats on the importin α surface.

* '''A 50kDa C-terminal''' <scene name='Sandbox_Reserved_709/Importin_nlsbs/1'>NLS binding sites</scene> contained in <scene name='Sandbox_Reserved_709/Importin_arm/3'>10 tandem armadillo (Arm) repeats</scene>. These arm repeat domains have an elongated superhelical structure and each of them contains 3 α-helices (H1, H2, H3). Together, H3 helices define the inner concave surface of the protein and the NLS-binding site. <ref>PMID:22248489</ref> The main chain of the cNLs runs antiparallel to the direction of the importin α superhelix, with the cNLS backbone interacting with an array of conserved asparagine residues in consecutive Arm repeats on the importin α surface.In fact there are two NLS-binding sites : a major one and a minor one. Thus, monopartite cNLSs are able to bind at two distinct sites on importin α (with a preference for the major site). Bipartite cNLSs simultaneously interact with both binding sites, with the larger C-terminal basic cluster binding to the major site (located in Arm repeats 2-4) and the smaller upstream cluster binding to the minor site (located in Arm repeats 6-8).

Here you can see a <scene name='Sandbox_Reserved_709/Nls_basic/1'>NLS </scene>bound to the NLS-binding site of importin α.

Sandbox Reserved 709: Difference between revisions

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@@ Line 52: / Line 52: @@
 Here, as the structure begins at the amino acid number 70, we cannot see the IBB which is located in the 2-60 region.
-* '''A 50kDa C-terminal''' <scene name='Sandbox_Reserved_709/Importin_nlsbs/1'>NLS binding sites</scene> contained in <scene name='Sandbox_Reserved_709/Importin_arm/3'>10 tandem armadillo (Arm) repeats</scene>. These arm repeat domains have an elongated superhelical structure and each of them contains 3 α-helices (H1, H2, H3). Together, H3 helices define the inner concave surface of the protein and the NLS-binding sites. Indeed, in fact there are two NLS-binding sites : a major one and a minor one (determined by their affinity for NLS). <ref>PMID:22248489</ref> The main chain of the cNLs runs antiparallel to the direction of the importin α superhelix, with the cNLS backbone interacting with an array of conserved asparagine residues in consecutive Arm repeats on the importin α surface.
+* '''A 50kDa C-terminal''' <scene name='Sandbox_Reserved_709/Importin_nlsbs/1'>NLS binding sites</scene> contained in <scene name='Sandbox_Reserved_709/Importin_arm/3'>10 tandem armadillo (Arm) repeats</scene>. These arm repeat domains have an elongated superhelical structure and each of them contains 3 α-helices (H1, H2, H3). Together, H3 helices define the inner concave surface of the protein and the NLS-binding site.  <ref>PMID:22248489</ref> The main chain of the cNLs runs antiparallel to the direction of the importin α superhelix, with the cNLS backbone interacting with an array of conserved asparagine residues in consecutive Arm repeats on the importin α surface.In fact there are two NLS-binding sites : a major one and a minor one. Thus, monopartite cNLSs are able to bind at two distinct sites on importin α (with a preference for the major site). Bipartite cNLSs simultaneously interact with both binding sites, with the larger C-terminal basic cluster binding to the major site (located in Arm repeats 2-4) and the smaller upstream cluster binding to the minor site (located in Arm repeats 6-8).
 Here you can see a <scene name='Sandbox_Reserved_709/Nls_basic/1'>NLS </scene>bound to the NLS-binding site of importin α.