Sandbox Reserved 709: Difference between revisions

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==Importin α : Nup50 complex==

''(<scene name='Sandbox_Reserved_709/Impnup_default/2'>Back to Default scene </scene>)''

Nup50 (''Nucleoporin 50 kDa'', about 450 aminoacids) is a part of the NPCs, in the nucleoplasmic side. It is able to bind to <scene name='Sandbox_Reserved_709/Importin/1'> importin α</scene>thanks to its <scene name='Sandbox_Reserved_709/Nup50/1'>N-terminus </scene> (''only 40 first aminoacids represented on the structure''). There are two binding sites for Nup50 on importin α :

* The first one overlaps with the minor NLS binding site. This binding is allowed by the two basic aminoacids<scene name='Sandbox_Reserved_709/Nup_bindingnls/1'>Lys3 and Arg4</scene> of Nup50. In fact, the positive charge will establish bonds with acidic aminoacids of the NLS binding site, just as an NLS-containing cargo would do. In presence of Nup50, the complex importin α:NLS dissociation rate is higher than the one of spontaneous dissociation : there is a competition between Nup50 and NLS, whose affinities for the NLS minor binding site are in the same order of magnitude.

* The second one binds to the C-terminus of importin α.

Nup50 (''Nucleoporin 50 kDa'', about 450 aminoacids) is a part of the NPCs, in the nucleoplasmic side. It is able to bind to <scene name='Sandbox_Reserved_709/Importin/1'> importin α</scene>thanks to its <scene name='Sandbox_Reserved_709/Nup50/1'>N-terminal domain</scene> (''only represented on the structure''). There are two binding sites for Nup50 on importin α :

* The first one overlaps with the minor NLS binding site. The affinity of Nup50 for this site is... <scene name='Sandbox_Reserved_709/Nup_bindingnls/1'>Lys3 and Arg4</scene>

==See Also==

Sandbox Reserved 709: Difference between revisions

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 ==Importin α : Nup50 complex==
-<Structure load='2c1m' size='300' color='white' frame='true' align='right' caption='Complex between mouse importin α(residues 70-529) and Nup50(or Npap60)' />
+<Structure load='2c1m' size='300' color='white' frame='true' align='right' caption='Complex between mouse importin α(residues 70-529) and Nup50(or Npap60)/>
+''(<scene name='Sandbox_Reserved_709/Impnup_default/2'>Back to Default scene </scene>)''
+Nup50 (''Nucleoporin 50 kDa'', about 450 aminoacids) is a part of the NPCs, in the nucleoplasmic side. It is able to bind to <scene name='Sandbox_Reserved_709/Importin/1'> importin α</scene>thanks to its <scene name='Sandbox_Reserved_709/Nup50/1'>N-terminus </scene> (''only 40 first aminoacids represented on the structure''). There are two binding sites for Nup50 on importin α :
+* The first one overlaps with the minor NLS binding site. This binding is allowed by the two basic aminoacids<scene name='Sandbox_Reserved_709/Nup_bindingnls/1'>Lys3 and Arg4</scene> of Nup50. In fact, the positive charge will establish bonds with acidic aminoacids of the NLS binding site, just as an NLS-containing cargo would do. In presence of Nup50, the complex importin α:NLS dissociation rate is higher than the one of spontaneous dissociation : there is a competition between Nup50 and NLS, whose affinities for the NLS minor binding site are in the same order of magnitude.
+* The second one binds to the C-terminus of importin α.
-Nup50 (''Nucleoporin 50 kDa'', about 450 aminoacids) is a part of the NPCs, in the nucleoplasmic side. It is able to bind to <scene name='Sandbox_Reserved_709/Importin/1'> importin α</scene>thanks to its <scene name='Sandbox_Reserved_709/Nup50/1'>N-terminal domain</scene> (''only represented on the structure''). There are two binding sites for Nup50 on importin α :
-* The first one overlaps with the minor NLS binding site. The affinity of Nup50 for this site is... <scene name='Sandbox_Reserved_709/Nup_bindingnls/1'>Lys3 and Arg4</scene>
 ==See Also==