1u12: Difference between revisions
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[[Image:1u12.gif|left|200px]] | [[Image:1u12.gif|left|200px]] | ||
'''M. loti cyclic nucleotide binding domain mutant''' | {{Structure | ||
|PDB= 1u12 |SIZE=350|CAPTION= <scene name='initialview01'>1u12</scene>, resolution 2.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''M. loti cyclic nucleotide binding domain mutant''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1U12 is a [ | 1U12 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti_maff303099 Mesorhizobium loti maff303099]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U12 OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel., Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH, Cell. 2004 Nov 24;119(5):615-27. PMID:[http:// | Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel., Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH, Cell. 2004 Nov 24;119(5):615-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15550244 15550244] | ||
[[Category: Mesorhizobium loti maff303099]] | [[Category: Mesorhizobium loti maff303099]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: unliganded]] | [[Category: unliganded]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:25:54 2008'' | ||
Revision as of 12:25, 20 March 2008
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| 1u12, resolution 2.70Å | |||||||||||||
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| Ligands: | IOD, SO4 and K | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
M. loti cyclic nucleotide binding domain mutant
Overview
Here we describe the initial functional characterization of a cyclic nucleotide regulated ion channel from the bacterium Mesorhizobium loti and present two structures of its cyclic nucleotide binding domain, with and without cAMP. The domains are organized as dimers with the interface formed by the linker regions that connect the nucleotide binding pocket to the pore domain. Together, structural and functional data suggest the domains form two dimers on the cytoplasmic face of the channel. We propose a model for gating in which ligand binding alters the structural relationship within a dimer, directly affecting the position of the adjacent transmembrane helices.
About this Structure
1U12 is a Single protein structure of sequence from Mesorhizobium loti maff303099. Full crystallographic information is available from OCA.
Reference
Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel., Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH, Cell. 2004 Nov 24;119(5):615-27. PMID:15550244
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