1wac: Difference between revisions

Line 1:

[[~~Image~~:~~1wac~~.~~png~~|~~left~~|~~200px~~]]

==BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE==

== Structural highlights ==

<table><tr><td colspan='2'>[[1wac]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAC FirstGlance]. <br>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wac RCSB], [http://www.ebi.ac.uk/pdbsum/1wac PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.

~~{{STRUCTURE_1wac| PDB=1wac | SCENE= }}~~

Back-priming mode of phi6 RNA-dependent RNA polymerase.,Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773<ref>PMID:15659773</ref>

~~===BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_15659773}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[1wac]] is a 3 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].~~

</StructureSection>

[[Category: Pseudomonas phage phi6]]

[[Category: Bamford, D H.]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1wac.png|left|200px]]
+==BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE==
+<StructureSection load='1wac' size='340' side='right' caption='[[1wac]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1wac]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAC FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hhs|1hhs]], [[1hht|1hht]], [[1hi0|1hi0]], [[1hi1|1hi1]], [[1hi8|1hi8]], [[1uvi|1uvi]], [[1uvj|1uvj]], [[1uvk|1uvk]], [[1uvl|1uvl]], [[1uvm|1uvm]], [[1uvn|1uvn]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wac OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wac RCSB], [http://www.ebi.ac.uk/pdbsum/1wac PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.
-{{STRUCTURE_1wac|  PDB=1wac  |  SCENE=  }}
+Back-priming mode of phi6 RNA-dependent RNA polymerase.,Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773<ref>PMID:15659773</ref>
-===BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_15659773}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1wac]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
+</StructureSection>
 [[Category: Pseudomonas phage phi6]]
 [[Category: Bamford, D H.]]