Sandbox Reserved 704: Difference between revisions

Line 15:

Leucyl-tRNA synthetase from the Archaeon ''Pyrococcus horikoshii'' is large (967 residues - 113kDa) and monomeric <ref name="Fukunaga">PMID:15663927</ref>. LeuRS catalyses the esterification of tRNAs Leu with Leucine. There are two classes of aaRSs. Leucyl-tRNA synthetase (LeuRS) belongs to the class I and more precisely to the class Ia. The class I enzymes have the Rossmann-fold domain (parallel β-sheet and α-helices) and the two characteristic motifs, with the consensus sequences of His-Ile-Gly-His (HIGH) and Lys-Met-Ser-Lys-Ser (KMSKS). This family is divided into prokaryotic and eukaryal/archaeal groups but we will focus on the second group.

Sandbox Reserved 704: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

Revision as of 12:25, 5 January 2013 view source Perrine Faiderbe (talk \| contribs) 45 edits No edit summary ← Older edit		Revision as of 12:26, 5 January 2013 view source Perrine Faiderbe (talk \| contribs) 45 edits No edit summary Newer edit →
Line 15:		Line 15:
	Leucyl-tRNA synthetase from the Archaeon ''Pyrococcus horikoshii'' is large (967 residues - 113kDa) and monomeric <ref name="Fukunaga">PMID:15663927</ref>. LeuRS catalyses the esterification of tRNAs Leu with Leucine. There are two classes of aaRSs. Leucyl-tRNA synthetase (LeuRS) belongs to the class I and more precisely to the class Ia. The class I enzymes have the Rossmann-fold domain (parallel β-sheet and α-helices) and the two characteristic motifs, with the consensus sequences of His-Ile-Gly-His (HIGH) and Lys-Met-Ser-Lys-Ser (KMSKS). This family is divided into prokaryotic and eukaryal/archaeal groups but we will focus on the second group.		Leucyl-tRNA synthetase from the Archaeon ''Pyrococcus horikoshii'' is large (967 residues - 113kDa) and monomeric <ref name="Fukunaga">PMID:15663927</ref>. LeuRS catalyses the esterification of tRNAs Leu with Leucine. There are two classes of aaRSs. Leucyl-tRNA synthetase (LeuRS) belongs to the class I and more precisely to the class Ia. The class I enzymes have the Rossmann-fold domain (parallel β-sheet and α-helices) and the two characteristic motifs, with the consensus sequences of His-Ile-Gly-His (HIGH) and Lys-Met-Ser-Lys-Ser (KMSKS). This family is divided into prokaryotic and eukaryal/archaeal groups but we will focus on the second group.

	<Structure load='1wkb' size='~~350~~' frame='true' align='right' caption='Crystal Structure of Leucyl-tRNA Synthetase from the Archaeon Pyrococcus horikoshii [[resolution 2.05Å]] (PDB entry : 1wkb) ' scene='Insert optional scene name here' />		<Structure load='1wkb' size='340' frame='true' align='right' caption='Crystal Structure of Leucyl-tRNA Synthetase from the Archaeon Pyrococcus horikoshii [[resolution 2.05Å]] (PDB entry : 1wkb) ' scene='Insert optional scene name here' />