1uhy: Difference between revisions
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'''Crystal structure of d(GCGATAGC): the base-intercalated duplex''' | {{Structure | ||
|PDB= 1uhy |SIZE=350|CAPTION= <scene name='initialview01'>1uhy</scene>, resolution 1.7Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of d(GCGATAGC): the base-intercalated duplex''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1UHY is a [ | 1UHY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UHY OCA]. | ||
==Reference== | ==Reference== | ||
X-ray analyses of d(GCGAXAGC) containing G and T at X: the base-intercalated duplex is still stable even in point mutants at the fifth residue., Kondo J, Umeda S, Fujita K, Sunami T, Takenaka A, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):117-20. Epub 2003 Nov 28. PMID:[http:// | X-ray analyses of d(GCGAXAGC) containing G and T at X: the base-intercalated duplex is still stable even in point mutants at the fifth residue., Kondo J, Umeda S, Fujita K, Sunami T, Takenaka A, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):117-20. Epub 2003 Nov 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14646150 14646150] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Fujita, K.]] | [[Category: Fujita, K.]] | ||
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[[Category: sheared g:a pair]] | [[Category: sheared g:a pair]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:32:17 2008'' | ||
Revision as of 12:32, 20 March 2008
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| 1uhy, resolution 1.7Å | |||||||||||||
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| Ligands: | NA, CL and NCO | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of d(GCGATAGC): the base-intercalated duplex
Overview
DNA fragments containing the sequence d(GCGAAAGC) prefer to adopt a base-intercalated (zipper-like) duplex in the crystalline state. To investigate effects of point mutation at the 5th residue on the structure, two crystal structures of d(GCGAGAGC) and d(GCGATAGC) have been determined by X-ray crystallography. In the respective crystals, the two octamers related by a crystallographic two-fold symmetry are aligned in an anti-parallel fashion and associated to each other to form a duplex, suggesting that the base-intercalated duplex is stable even when the 5th residue is mutated with other bases. The sheared G3:A6 pair formation makes the two phosphate backbones closer and facilitates formation of the A-X*-X-A* base-intercalated motif. The three duplexes are assembled around the three-fold axis, and their 3rd and 4th residues are bound to the hexamine cobalt chloride. The central 5th residues are bound to another cation.
About this Structure
1UHY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
X-ray analyses of d(GCGAXAGC) containing G and T at X: the base-intercalated duplex is still stable even in point mutants at the fifth residue., Kondo J, Umeda S, Fujita K, Sunami T, Takenaka A, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):117-20. Epub 2003 Nov 28. PMID:14646150
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