Sandbox Reserved 707: Difference between revisions
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'''The complex of wild type B-RAF and BAY439006''' | '''The complex of wild type B-RAF and BAY439006''' | ||
{{STRUCTURE_1uwh| PDB=1uwh | SCENE= |left}} | |||
[[Image:1uwh bio r 500.jpg|300px|thumb| The complex of wild type B-RAF and BAY439006 ([[1uwh]])]] | [[Image:1uwh bio r 500.jpg|300px|thumb| The complex of wild type B-RAF and BAY439006 ([[1uwh]])]] | ||
== Introduction == | == Introduction == | ||
The B-RAF Protein belongs to a large family of protein kinases that have a very important role on different aspects of cellular biology and biomedical sciences. The human protein kinase family consists of 518 genes, making it one of the largest gene families. Based upon the phosphorylation of the hydroxyl group these proteins are classified on 3 groups: protein serine threonine kinase (385 members), protein tyrosine kinase (90 members) and tyrosine kinase like proteins (43 members). A-RAF, B-RAF and C-RAF proteins are a family of serine threonine kinase proteins that participate in the RAS-RAF-MEK-ERK signal transduction cascade. The general reaction they catalyse is: | The B-RAF Protein belongs to a large family of protein kinases that have a very important role on different aspects of cellular biology and biomedical sciences. The human protein kinase family consists of 518 genes, making it one of the largest gene families. Based upon the phosphorylation of the hydroxyl group these proteins are classified on 3 groups: protein serine threonine kinase (385 members), protein tyrosine kinase (90 members) and tyrosine kinase like proteins (43 members). A-RAF, B-RAF and C-RAF proteins are a family of serine threonine kinase proteins that participate in the RAS-RAF-MEK-ERK signal transduction cascade. The general reaction they catalyse is: | ||
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== B-RAF Structure<ref>PMID: 15035987</ref> == | == B-RAF Structure<ref>PMID: 15035987</ref> == | ||
[[Image:1-s2.0-S0092867404002156-main page7 image1.jpg|400px|thumb| A. Structure of B-RAF Kinase Domain <br /> | [[Image:1-s2.0-S0092867404002156-main page7 image1.jpg|400px|thumb| A. Structure of B-RAF Kinase Domain <br /> | ||
B. Schematic B-RAF Primary Structure]] | B. Schematic B-RAF Primary Structure]] | ||
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A <scene name='Sandbox_Reserved_707/Gatekeeper_residue/1'>gatekeeper residue</scene> separates the adenine binding site from the hydrophobic pocket. Mutation on this residue can prevent the binding of kinase inhibitory drugs (the replacement of a threonine by a methionine for example).<br /> | A <scene name='Sandbox_Reserved_707/Gatekeeper_residue/1'>gatekeeper residue</scene> separates the adenine binding site from the hydrophobic pocket. Mutation on this residue can prevent the binding of kinase inhibitory drugs (the replacement of a threonine by a methionine for example).<br /> | ||
<Structure load='1UWH' size='400' frame='true' align='left' caption='3D View of the complex ; resolution 2,95 A' scene='Insert optional scene name here' /> | |||
It is very important to distinguish conserved amino acid residue signatures that constitute the catalytic core of the B-RAF Kinase. The catalytic properties are done by the <scene name='Sandbox_Reserved_707/Kdd_motif/1'>KDD (Lys578-Asp576-Asp594)</scene> Motif and the <scene name='Sandbox_Reserved_707/Activation_segment/1'>activation segment</scene> starts in general with a <scene name='Sandbox_Reserved_707/Dfg_start_activation_segment/1'>DFG</scene> and ends with <scene name='Sandbox_Reserved_707/Ape/1'>APE</scene>. But how the catalytic reaction is generated? | It is very important to distinguish conserved amino acid residue signatures that constitute the catalytic core of the B-RAF Kinase. The catalytic properties are done by the <scene name='Sandbox_Reserved_707/Kdd_motif/1'>KDD (Lys578-Asp576-Asp594)</scene> Motif and the <scene name='Sandbox_Reserved_707/Activation_segment/1'>activation segment</scene> starts in general with a <scene name='Sandbox_Reserved_707/Dfg_start_activation_segment/1'>DFG</scene> and ends with <scene name='Sandbox_Reserved_707/Ape/1'>APE</scene>. But how the catalytic reaction is generated? | ||
