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[[Image:1zmo.png|left|200px]]
==Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2==
<StructureSection load='1zmo' size='340' side='right' caption='[[1zmo]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1zmo]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_sp._ad2 Arthrobacter sp. ad2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZMO FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zmt|1zmt]], [[1zo8|1zo8]]</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zmo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zmo RCSB], [http://www.ebi.ac.uk/pdbsum/1zmo PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zm/1zmo_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Haloalcohol dehalogenases are bacterial enzymes that cleave the carbon-halogen bond in short aliphatic vicinal haloalcohols, like 1-chloro-2,3-propanediol, some of which are recalcitrant environmental pollutants. They use a conserved Ser-Tyr-Arg catalytic triad to deprotonate the haloalcohol oxygen, which attacks the halogen-bearing carbon atom, producing an epoxide and a halide ion. Here, we present the X-ray structure of the haloalcohol dehalogenase HheA(AD2) from Arthrobacter sp. strain AD2 at 2.0-A resolution. Comparison with the previously reported structure of the 34% identical enantioselective haloalcohol dehalogenase HheC from Agrobacterium radiobacter AD1 shows that HheA(AD2) has a similar quaternary and tertiary structure but a much more open substrate-binding pocket. Docking experiments reveal that HheA(AD2) can bind both enantiomers of the haloalcohol substrate 1-p-nitrophenyl-2-chloroethanol in a productive way, which explains the low enantiopreference of HheA(AD2). Other differences are found in the halide-binding site, where the side chain amino group of Asn182 is in a position to stabilize the halogen atom or halide ion in HheA(AD2), in contrast to HheC, where a water molecule has taken over this role. These results broaden the insight into the structural determinants that govern reactivity and selectivity in the haloalcohol dehalogenase family.


{{STRUCTURE_1zmo|  PDB=1zmo  |  SCENE=  }}
The X-ray structure of the haloalcohol dehalogenase HheA from Arthrobacter sp. strain AD2: insight into enantioselectivity and halide binding in the haloalcohol dehalogenase family.,de Jong RM, Kalk KH, Tang L, Janssen DB, Dijkstra BW J Bacteriol. 2006 Jun;188(11):4051-6. PMID:16707696<ref>PMID:16707696</ref>


===Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


{{ABSTRACT_PUBMED_16707696}}
==See Also==
 
*[[Dehalogenase|Dehalogenase]]
==About this Structure==
== References ==
[[1zmo]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arthrobacter_sp._ad2 Arthrobacter sp. ad2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZMO OCA].
<references/>
 
__TOC__
==Reference==
</StructureSection>
<ref group="xtra">PMID:016707696</ref><references group="xtra"/>
[[Category: Arthrobacter sp. ad2]]
[[Category: Arthrobacter sp. ad2]]
[[Category: Dijkstra, B W.]]
[[Category: Dijkstra, B W.]]
Retrieved from "https://proteopedia.org/1zmo"