Sandbox Reserved 707: Difference between revisions
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|'''<scene name='Sandbox_Reserved_707/Cr3_domain/2'>CR3, protein kinase domain</scene>''': || 451-717 | |'''<scene name='Sandbox_Reserved_707/Cr3_domain/2'>CR3, protein kinase domain</scene>''': || 451-717 | ||
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|'''<scene name='Sandbox_Reserved_707/Glycine_rich_loop/1'>Glycine Rich Loop</scene>''': || 463-471 | |'''<scene name='Sandbox_Reserved_707/Glycine_rich_loop/1'>Glycine Rich Loop</scene>'''<ref>PMID:12068308</ref>: || 463-471 | ||
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|'''14-3-3 binding site''': || S365-S729 | |'''14-3-3 binding site''': || S365-S729 | ||
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Under non stimulatory conditions a serine of CR2 domain and another near the C-terminus are phosphorylated and are bound to 14-3-3 domain. To activate RAF Kinases, RAS-GTP has to interact with the RDB Domain. This is necessary but not enough to activate the RAF Kinase. | Under non stimulatory conditions a serine of CR2 domain and another near the C-terminus are phosphorylated and are bound to 14-3-3 domain. To activate RAF Kinases, RAS-GTP has to interact with the RDB Domain. This is necessary but not enough to activate the RAF Kinase. | ||
One characteristic that distinguish B-RAF from A or C-RAF is the presence of Asp448 and Asp449 on the N-terminal region, which bear negative charges. Phosphorylation of <scene name='Sandbox_Reserved_707/Psite_of_activation_segment/1'>Thr599</scene> and Ser602 in the activation segment is essential for B-RAF activation | One characteristic that distinguish B-RAF from A or C-RAF is the presence of Asp448 and Asp449 on the N-terminal region, which bear negative charges. Phosphorylation of <scene name='Sandbox_Reserved_707/Psite_of_activation_segment/1'>Thr599</scene> and Ser602 in the activation segment is essential for B-RAF activation. That’s why the basal activity of B-RAF is greater than A or C-RAF.<br /> | ||
