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=== Two magnesium ions are coordinated by the catamytic site ===
=== Two magnesium ions are coordinated by the catalytic site ===
Adenylyl cyclase activity of the edema factor requires two magnesium ions. One of them is coordinated by two aspartic residues (D491 and D493) and the histidine 577. The other one is coordianted by the non-esterified oxygens of α, β and γ phosphates of ATP and by aspartic D493. The distance between these two magnesium ions is about 4.5 Å.