Sandbox Reserved 707: Difference between revisions
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[[Image:1-s2.0-S0092867404002156-main page7 image1.jpg|400px|thumb| A. Structure of B-RAF Kinase Domain <br /> | [[Image:1-s2.0-S0092867404002156-main page7 image1.jpg|400px|thumb| A. Structure of B-RAF Kinase Domain <br /> | ||
B. Schematic B-RAF Primary Structure]] | B. Schematic B-RAF Primary Structure<ref>PMID: 15035987</ref>]] | ||
As all RAF Proteins, B-RAF has the characteristic <scene name='Sandbox_Reserved_707/Small_lobe/1'>small N-terminal lobe</scene> and the <scene name='Sandbox_Reserved_707/Large_lobe/1'>large C-terminal lobe</scene>. | As all RAF Proteins, B-RAF has the characteristic <scene name='Sandbox_Reserved_707/Small_lobe/1'>small N-terminal lobe</scene> and the <scene name='Sandbox_Reserved_707/Large_lobe/1'>large C-terminal lobe</scene>. | ||
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An invariant lysine (<scene name='Sandbox_Reserved_707/K578/1'>Lys578</scene> in B-RAF) forms salt bridges with the gamma phosphate of the ATP. Asp576, which is a base in the catalytic loop, orients the seryl or threonyl group of the substrate protein and takes the proton of the hydroxyl group, facilitating the attack of oxygen on the gamma phosphorus atom of MgATP. Asp594 binds Mg²⁺ which coordinates the beta and gamma phosphates of ATP. <br/> | An invariant lysine (<scene name='Sandbox_Reserved_707/K578/1'>Lys578</scene> in B-RAF) forms salt bridges with the gamma phosphate of the ATP. Asp576, which is a base in the catalytic loop, orients the seryl or threonyl group of the substrate protein and takes the proton of the hydroxyl group, facilitating the attack of oxygen on the gamma phosphorus atom of MgATP. Asp594 binds Mg²⁺ which coordinates the beta and gamma phosphates of ATP. <br/> | ||
[[Image:138 page3 image1.jpg|500px|thumb| Diagram of the inferred interactions between human B-RAF kinase catalytic core residues, ATP, and MEK]] | [[Image:138 page3 image1.jpg|500px|thumb| Diagram of the inferred interactions between human B-RAF kinase catalytic core residues, ATP, and MEK<ref>PMID:22569528</ref>]] | ||
This is a table of the important residues of B-RAF:<br /> | This is a table of the important residues of B-RAF:<br /> | ||
