1gql: Difference between revisions
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==Overview== | ==Overview== | ||
Alpha-glucuronidases, components of an ensemble of enzymes central to the, recycling of photosynthetic biomass, remove the alpha-1,2 linked, 4-O-methyl glucuronic acid from xylans. The structure of the, alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three, domains, the central of which is a (beta/alpha)(8) barrel housing the, catalytic apparatus. Complexes of the enzyme with the individual reaction, products, either xylobiose or glucuronic acid, and the ternary complex of, both glucuronic acid and xylotriose reveal a "blind" pocket which selects, for short decorated xylooligosaccharides substituted with the uronic acid, at their nonreducing end, consistent with kinetic data. The catalytic, center reveals a constellation of carboxylates; Glu292 is poised to, provide . | Alpha-glucuronidases, components of an ensemble of enzymes central to the, recycling of photosynthetic biomass, remove the alpha-1,2 linked, 4-O-methyl glucuronic acid from xylans. The structure of the, alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three, domains, the central of which is a (beta/alpha)(8) barrel housing the, catalytic apparatus. Complexes of the enzyme with the individual reaction, products, either xylobiose or glucuronic acid, and the ternary complex of, both glucuronic acid and xylotriose reveal a "blind" pocket which selects, for short decorated xylooligosaccharides substituted with the uronic acid, at their nonreducing end, consistent with kinetic data. The catalytic, center reveals a constellation of carboxylates; Glu292 is poised to, provide protonic assistance to leaving group departure with Glu393 and, Asp365 both appropriately positioned to provide base-catalyzed assistance, for inverting nucleophilic attack by water. | ||
==About this Structure== | ==About this Structure== | ||
1GQL is a | 1GQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus] with BDP, CO and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-glucuronidase Alpha-glucuronidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.139 3.2.1.139] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GQL OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: xylotriose]] | [[Category: xylotriose]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 15:32:28 2007'' | ||
