1grq: Difference between revisions
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==Overview== | ==Overview== | ||
Streptomyces venezuelae synthesizes chloramphenicol (Cm), an inhibitor of, ribosomal peptidyl transferase activity, thereby inhibiting bacterial, growth. The producer escapes autoinhibition by its own secondary, metabolite through phosphorylation of Cm by chloramphenicol, phosphotransferase (CPT). In addition to active site binding, CPT binds, its product 3-phosphoryl-Cm, in an alternate product binding site. To, address the mechanisms of Cm tolerance of the producer, the crystal, structures of CPT were determined in complex with either the, nonchlorinated Cm (2-N-Ac-Cm) at 3.1 A resolution or the antibiotic's, immediate precursor, the p-amino analog p-NH(2)-Cm, at 2.9 A resolution., Surprisingly, p-NH(2)-Cm binds CPT in a novel fashion. Additionally, neither 2-N-Ac-Cm nor p-NH(2)-Cm . | Streptomyces venezuelae synthesizes chloramphenicol (Cm), an inhibitor of, ribosomal peptidyl transferase activity, thereby inhibiting bacterial, growth. The producer escapes autoinhibition by its own secondary, metabolite through phosphorylation of Cm by chloramphenicol, phosphotransferase (CPT). In addition to active site binding, CPT binds, its product 3-phosphoryl-Cm, in an alternate product binding site. To, address the mechanisms of Cm tolerance of the producer, the crystal, structures of CPT were determined in complex with either the, nonchlorinated Cm (2-N-Ac-Cm) at 3.1 A resolution or the antibiotic's, immediate precursor, the p-amino analog p-NH(2)-Cm, at 2.9 A resolution., Surprisingly, p-NH(2)-Cm binds CPT in a novel fashion. Additionally, neither 2-N-Ac-Cm nor p-NH(2)-Cm binds to the secondary product binding, site. | ||
==About this Structure== | ==About this Structure== | ||
1GRQ is a | 1GRQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_venezuelae Streptomyces venezuelae] with SO4 and CLK as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: SO4. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GRQ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:43:36 2007'' | ||
