1guv: Difference between revisions
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==Overview== | ==Overview== | ||
Chitin hydrolases have been identified in a variety of organisms ranging, from bacteria to eukaryotes. They have been proposed to be possible, targets for the design of novel chemotherapeutics against human pathogens, such as fungi and protozoan parasites as mammals were not thought to, possess chitin-processing enzymes. Recently, a human chitotriosidase was, described as a marker for Gaucher disease with plasma levels of the enzyme, elevated up to 2 orders of magnitude. The chitotriosidase was shown to be, active against colloidal chitin and is inhibited by the family 18, chitinase inhibitor allosamidin. Here, the crystal structure of the human, chitotriosidase and complexes with a chitooligosaccharide and allosamidin, are described. The structures reveal an elongated active site cleft, ... | Chitin hydrolases have been identified in a variety of organisms ranging, from bacteria to eukaryotes. They have been proposed to be possible, targets for the design of novel chemotherapeutics against human pathogens, such as fungi and protozoan parasites as mammals were not thought to, possess chitin-processing enzymes. Recently, a human chitotriosidase was, described as a marker for Gaucher disease with plasma levels of the enzyme, elevated up to 2 orders of magnitude. The chitotriosidase was shown to be, active against colloidal chitin and is inhibited by the family 18, chitinase inhibitor allosamidin. Here, the crystal structure of the human, chitotriosidase and complexes with a chitooligosaccharide and allosamidin, are described. The structures reveal an elongated active site cleft, compatible with the binding of long chitin polymers, and explain the, inactivation of the enzyme through an inherited genetic deficiency., Comparison with YM1 and HCgp-39 shows how the chitinase has evolved into, these mammalian lectins by the mutation of key residues in the active, site, tuning the substrate binding specificity. The soaking experiments, with allosamidin and chitooligosaccharides give insight into ligand, binding properties and allow the evaluation of differential binding and, design of species-selective chitinase inhibitors. | ||
==About this Structure== | ==About this Structure== | ||
1GUV is a | 1GUV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with EDO as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: EGA. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GUV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: lectin]] | [[Category: lectin]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:06:53 2007'' | ||
