Sandbox Reserved 706: Difference between revisions
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=== '''Structure of Rab27B''' === | === '''Structure of Rab27B''' === | ||
[[Image:Rab27B slac2a image1.jpg | 425 px | thumb | right | | [[Image:Rab27B slac2a image1.jpg | 425 px | thumb | right |Figure 2: Overall stucture of the Rab27B/Slac2-a complex. <ref>PMID:18940604</ref>]] | ||
'''General informations:''' | '''General informations:''' | ||
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Rab27B adopts a globular form in the complex. The | Rab27B adopts a globular form in the complex. The | ||
<scene name='Sandbox_Reserved_706/Loop_entre_beta_2_et_3/1'>ß2 and ß3 loop (residues 55-64)</scene> and <scene name='Sandbox_Reserved_706/Rab27b_loop_2_alpha3_b5/1'>α3-ß5 loop (residues122-126)</scene> of Rab27B are both regions involved in Slac2-a binding. <ref>PMID:18940604</ref> | <scene name='Sandbox_Reserved_706/Loop_entre_beta_2_et_3/1'>ß2 and ß3 loop (residues 55-64)</scene> and <scene name='Sandbox_Reserved_706/Rab27b_loop_2_alpha3_b5/1'>α3-ß5 loop (residues122-126)</scene> of Rab27B are both regions involved in Slac2-a binding. <ref>PMID:18940604</ref> | ||
[[Image:Rab27B slac2a image2.jpg | 450 px | thumb | left | | [[Image:Rab27B slac2a image2.jpg | 450 px | thumb | left |Figure 3: Structural comparaison of Rab27B in GTP and GDP form. <ref>PMID:18940604</ref>]] | ||
The complex Rab27B/Slac2-a can only be formed when Rab27B is in a GTP form. This can be explained because of the changing of shape between Rab27B in the GTP and GDP form. (See figure 3) | The complex Rab27B/Slac2-a can only be formed when Rab27B is in a GTP form. This can be explained because of the changing of shape between Rab27B in the GTP and GDP form. (See figure 3) | ||
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<scene name='Sandbox_Reserved_706/Cys_64_67_89_92/2'>Cys64, Cys67, Cys89 and Cys92</scene> for Zn1 binding, and <scene name='Sandbox_Reserved_706/Cys81_cys84_cys104_cys107/2'>Cys81, Cys84, Cys104, and Cys107</scene> for Zn2 binding. <ref>PMID:18940604</ref> | <scene name='Sandbox_Reserved_706/Cys_64_67_89_92/2'>Cys64, Cys67, Cys89 and Cys92</scene> for Zn1 binding, and <scene name='Sandbox_Reserved_706/Cys81_cys84_cys104_cys107/2'>Cys81, Cys84, Cys104, and Cys107</scene> for Zn2 binding. <ref>PMID:18940604</ref> | ||
<Structure load='2ZET' size='500' frame='true' align='right' caption='Final model of 2zet: Two Rab27B/Slac2-a complexes (A/C and B/D) | <Structure load='2ZET' size='500' frame='true' align='right' caption='Final model of 2zet: Two Rab27B/Slac2-a complexes (A/C and B/D)' scene='Insert optional scene name here' /> | ||
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Rab27B binds to Slac2-a with good shape complementarity and this interaction involves many hydrophobic interactions and several electrostatic interactions. | Rab27B binds to Slac2-a with good shape complementarity and this interaction involves many hydrophobic interactions and several electrostatic interactions. | ||
There are three contact areas in the interface between Rab27B and Slac2-a. The first and second contact areas involve the coiled-coil regions of Slac2-a and constitute the main interface. | There are three contact areas in the interface between Rab27B and Slac2-a. The first and second contact areas involve the coiled-coil regions of Slac2-a and constitute the main interface. | ||
[[Image:Rab27B slac2a image3.gif | | |||
[[Image:Rab27B slac2a image3.gif | 225 px | thumb | left |Figure 4: Rab27B/Slac2-a contact interfaces : | |||
(A) Contact area 1, | (A) Contact area 1, | ||
(C) Contact area 2, | (C) Contact area 2, | ||
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==Contributors== | =='''Contributors'''== | ||
Pernelle Klein, Myriam Deshaies | Pernelle Klein, Myriam Deshaies | ||
