Sandbox Reserved 706: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
|||
| Line 56: | Line 56: | ||
[[Image:Rab27B slac2a image2.jpg | 450 px | thumb | left |Figure 3: Structural comparaison of Rab27B in GTP and GDP form. <ref>PMID:18940604</ref>]] | [[Image:Rab27B slac2a image2.jpg | 450 px | thumb | left |Figure 3: Structural comparaison of Rab27B in GTP and GDP form. <ref>PMID:18940604</ref>]] | ||
The complex Rab27B/Slac2-a can only be formed when Rab27B is in a GTP form. This can be explained because of the changing of shape between Rab27B in the GTP and GDP form | The complex Rab27B/Slac2-a can only be formed when Rab27B is in a GTP form. This can be explained because of the changing of shape between Rab27B in the GTP and GDP form (see figure 3). | ||
Rab27B–GTP adopts a compact monomer conformation, while an extended conformation is observed for GDP-bound Rab27B. | Rab27B–GTP adopts a compact monomer conformation, while an extended conformation is observed for GDP-bound Rab27B. | ||
<scene name='Sandbox_Reserved_706/Switch_1_rab27b/1'>Switch 1</scene> and <scene name='Sandbox_Reserved_706/Switch2_rab27b/1'>switch 2</scene> of Rab27B play an important role in this changing of conformation. In fact, switch 1 and 2 allow GTP binding by making hydrogen bond with GTP's γ-phosphate, and this way also allows Slac2-a binding. But in the complex Rab27B/GDP switch 1 and switch 2 exist completely apart from the bound GDP, so there is no interaction with Slac2-a and no binding. | <scene name='Sandbox_Reserved_706/Switch_1_rab27b/1'>Switch 1</scene> and <scene name='Sandbox_Reserved_706/Switch2_rab27b/1'>switch 2</scene> of Rab27B play an important role in this changing of conformation. In fact, switch 1 and 2 allow GTP binding by making hydrogen bond with GTP's γ-phosphate, and this way also allows Slac2-a binding. But in the complex Rab27B/GDP switch 1 and switch 2 exist completely apart from the bound GDP, so there is no interaction with Slac2-a and no binding. | ||
=== '''Structure of Slac2-a''' === | === '''Structure of Slac2-a''' === | ||
