Sandbox Reserved 706: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
|||
| Line 2: | Line 2: | ||
{{Sandbox_ESBS_2012}} | {{Sandbox_ESBS_2012}} | ||
<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | <!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | ||
Please, do not delete or modify text or images, this page is reserved for a work from two students in ESBS. Thank you. | |||
<big>'''Crystal structure of the small GTPase Rab27B complexed with the Slp homology domain of Melanophilin'''</big> | |||
[[Image:2zet.png|left|200px]] {{STRUCTURE_2zet| PDB=2zet | SCENE= }} | |||
=='''Introduction'''== | =='''Introduction'''== | ||
| Line 59: | Line 61: | ||
Rab27B–GTP adopts a compact monomer conformation, while an extended conformation is observed for GDP-bound Rab27B. | Rab27B–GTP adopts a compact monomer conformation, while an extended conformation is observed for GDP-bound Rab27B. | ||
<scene name='Sandbox_Reserved_706/Switch_1_rab27b/1'>Switch 1</scene> and <scene name='Sandbox_Reserved_706/Switch2_rab27b/1'>switch 2</scene> of Rab27B play an important role in this changing of conformation. In fact, switch 1 and 2 allow GTP binding by making hydrogen bond with GTP's γ-phosphate, and this way also allows Slac2-a binding. But in the complex Rab27B/GDP switch 1 and switch 2 exist completely apart from the bound GDP, so there is no interaction with Slac2-a and no binding. | <scene name='Sandbox_Reserved_706/Switch_1_rab27b/1'>Switch 1</scene> and <scene name='Sandbox_Reserved_706/Switch2_rab27b/1'>switch 2</scene> of Rab27B play an important role in this changing of conformation. In fact, switch 1 and 2 allow GTP binding by making hydrogen bond with GTP's γ-phosphate, and this way also allows Slac2-a binding. But in the complex Rab27B/GDP switch 1 and switch 2 exist completely apart from the bound GDP, so there is no interaction with Slac2-a and no binding. | ||
=== '''Structure of Slac2-a''' === | === '''Structure of Slac2-a''' === | ||
