1gvn: Difference between revisions

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==Overview==

Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ..~~. [[http://ispc.weizmann.ac.il/pmbin/getpm?12571357 (full description)]]~~

Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ATPGTP-binding site. To our knowledge, this is the, first prokaryotic postsegregational killing system that has been entirely, structurally characterized.

==About this Structure==

1GVN is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: SOB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVN OCA]].

1GVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: SOB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVN OCA].

==Reference==

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[[Category: postsegregational killing system]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on ~~Tue Oct 30 15~~:20:36 2007''

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:45:06 2007''

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@@ Line 5: / Line 5: @@
 ==Overview==
-Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12571357 (full description)]]
+Programmed cell death in prokaryotes is frequently found as, postsegregational killing. It relies on antitoxin/toxin systems that, secure stable inheritance of low and medium copy number plasmids during, cell division and kill cells that have lost the plasmid. The, broad-host-range, low-copy-number plasmid pSM19035 from Streptococcus, pyogenes carries the genes encoding the antitoxin/toxin system, epsilon/zeta and antibiotic resistance proteins, among others. The crystal, structure of the biologically nontoxic epsilon(2)zeta(2) protein complex, at a 1.95-A resolution and site-directed mutagenesis showed that free zeta, acts as phosphotransferase by using ATPGTP. In epsilon(2)zeta(2), the, toxin zeta is inactivated because the N-terminal helix of the antitoxin, epsilon blocks the ATPGTP-binding site. To our knowledge, this is the, first prokaryotic postsegregational killing system that has been entirely, structurally characterized.
 ==About this Structure==
-GVN is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: SOB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVN OCA]].
+GVN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: SOB. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVN OCA].
 ==Reference==
@@ Line 22: / Line 22: @@
 [[Category: postsegregational killing system]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:20:36 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:45:06 2007''