1vyb: Difference between revisions
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[[Image:1vyb.jpg|left|200px]] | [[Image:1vyb.jpg|left|200px]] | ||
'''ENDONUCLEASE DOMAIN OF HUMAN LINE1 ORF2P''' | {{Structure | ||
|PDB= 1vyb |SIZE=350|CAPTION= <scene name='initialview01'>1vyb</scene>, resolution 1.80Å | |||
|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''ENDONUCLEASE DOMAIN OF HUMAN LINE1 ORF2P''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1VYB is a [ | 1VYB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VYB OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the targeting endonuclease of the human LINE-1 retrotransposon., Weichenrieder O, Repanas K, Perrakis A, Structure. 2004 Jun;12(6):975-86. PMID:[http:// | Crystal structure of the targeting endonuclease of the human LINE-1 retrotransposon., Weichenrieder O, Repanas K, Perrakis A, Structure. 2004 Jun;12(6):975-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15274918 15274918] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:49:40 2008'' | ||
Revision as of 12:49, 20 March 2008
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| 1vyb, resolution 1.80Å | |||||||||||||
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| Sites: | AC1 | ||||||||||||
| Ligands: | SO4, SO3 and GOL | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
ENDONUCLEASE DOMAIN OF HUMAN LINE1 ORF2P
Overview
The human L1 endonuclease (L1-EN) is encoded by the non-LTR retrotransposon LINE-1 (L1). L1 is responsible for more than 1.5 million retrotransposition events in the history of the human genome, contributing more than a quarter to human genomic DNA (L1 and Alu elements). L1-EN is related to the well-understood human DNA repair endonuclease APE1, and its nicking specificity is a major determinant for retrotransposon integration site selection. The crystal structure of human L1 endonuclease is the first of a retrotransposon-encoded protein and a prototype for retrotransposon-encoded endonucleases involved in target-primed reverse transcription. Structure-based endonuclease alignments reveal a conserved threonine in addition to previously identified invariant residues and suggest that DNA recognition proceeds via the accommodation of an extrahelical nucleotide within a pocket of the enzyme. The present analysis will help to refine phylogenetic and functional relationships among metal-dependent phosphohydrolases and provides a basis for manipulating non-LTR retrotransposon integration site selection.
About this Structure
1VYB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the targeting endonuclease of the human LINE-1 retrotransposon., Weichenrieder O, Repanas K, Perrakis A, Structure. 2004 Jun;12(6):975-86. PMID:15274918
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