1wmw: Difference between revisions

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[[Image:1wmw.gif|left|200px]]<br /><applet load="1wmw" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:1wmw.gif|left|200px]]
caption="1wmw, resolution 1.55&Aring;" />
 
'''Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus'''<br />
{{Structure
|PDB= 1wmw |SIZE=350|CAPTION= <scene name='initialview01'>1wmw</scene>, resolution 1.55&Aring;
|SITE=
|LIGAND=
|ACTIVITY=
|GENE=
}}
 
'''Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
1WMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA].  
1WMW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMW OCA].  


==Reference==
==Reference==
Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8., Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, Miura K, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):178-80. Epub 2003, Dec 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684922 14684922]
Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8., Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, Miura K, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):178-80. Epub 2003, Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684922 14684922]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: riken structural genomics/proteomics initiative]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: rsgi]]
[[Category: rsgi]]
[[Category: structural genomics]]
[[Category: structural genomic]]
[[Category: thermus]]
[[Category: thermus]]


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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:59:01 2008''

Revision as of 12:59, 20 March 2008

File:1wmw.gif


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1wmw, resolution 1.55Å
Coordinates: save as pdb, mmCIF, xml



Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus


Overview

Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 was expressed in Escherichia coli, purified to homogeneity and crystallized both as the recombinant native protein and its selenomethionine (SeMet) derivative. Well diffracting crystals of these proteins were obtained belonging to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8.

About this Structure

1WMW is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8., Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, Miura K, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):178-80. Epub 2003, Dec 18. PMID:14684922

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