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[[Image:2jtc.png|left|200px]]
==3D structure and backbone dynamics of SPE B==
<StructureSection load='2jtc' size='340' side='right' caption='[[2jtc]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2jtc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_m1 Streptococcus pyogenes serotype m1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JTC FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">speB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301447 Streptococcus pyogenes serotype M1])</td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Streptopain Streptopain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.10 3.4.22.10] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jtc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jtc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jtc RCSB], [http://www.ebi.ac.uk/pdbsum/2jtc PDBsum]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/2jtc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Streptococcal pyrogenic exotoxin B (SPE B) is a cysteine protease expressed by Streptococcus pyogenes. The D9N, G163S, G163S/A172S, and G239D mutant proteins were expressed to study the effect of the allelic variants on their protease activity. In contrast to other mutants, the G239D mutant was approximately 12-fold less active. The Gly-239 residue is located within the C-terminal S230-G239 region, which cannot be observed in the x-ray structure. The three-dimensional structure and backbone dynamics of the 28-kDa mature SPE B (mSPE B) were determined. Unlike the x-ray structure of the 40-kDa zymogen SPE B (proSPE B), we observed the interactions between the C-terminal loop and the active site residues in mSPE B. The structural differences between mSPE B and proSPE B were the conformation of the C-terminal loop and the orientation of the catalytic His-195 residue, suggesting that activation and inactivation of SPE B is involved in the His-195 side-chain rotation. Dynamics analysis of mSPE B and the mSPE B/inhibitor complexes showed that the catalytic and C-terminal loops were the most flexible regions with low order parameter values of 0.5 to 0.8 and exhibited the motion on the ps/ns timescale. These findings suggest that the flexible C-terminal loop of SPE B may play an important role in controlling the substrate binding, resulting in its broad substrate specificity.


{{STRUCTURE_2jtc|  PDB=2jtc  |  SCENE=  }}
Solution structure and backbone dynamics of streptopain: insight into diverse substrate specificity.,Wang CC, Houng HC, Chen CL, Wang PJ, Kuo CF, Lin YS, Wu JJ, Lin MT, Liu CC, Huang W, Chuang WJ J Biol Chem. 2009 Apr 17;284(16):10957-67. Epub 2009 Feb 23. PMID:19237546<ref>PMID:19237546</ref>


===3D structure and backbone dynamics of SPE B===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


{{ABSTRACT_PUBMED_19237546}}
==See Also==
 
*[[Exotoxin|Exotoxin]]
==About this Structure==
== References ==
[[2jtc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_m1 Streptococcus pyogenes serotype m1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JTC OCA].
<references/>
 
__TOC__
==Reference==
</StructureSection>
<ref group="xtra">PMID:019237546</ref><references group="xtra"/>
[[Category: Streptococcus pyogenes serotype m1]]
[[Category: Streptococcus pyogenes serotype m1]]
[[Category: Streptopain]]
[[Category: Streptopain]]
Retrieved from "https://proteopedia.org/2jtc"