2jlt: Difference between revisions
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[[ | ==CRYSTAL STRUCTURE OF AN RNA KISSING COMPLEX== | ||
<StructureSection load='2jlt' size='340' side='right' caption='[[2jlt]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2jlt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JLT FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5BU:5-BROMO-URIDINE-5-MONOPHOSPHATE'>5BU</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jlt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jlt RCSB], [http://www.ebi.ac.uk/pdbsum/2jlt PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In HIV-1, trans-activation of transcription of the viral genome is regulated by an imperfect hairpin, the trans-activating responsive (TAR) RNA element, located at the 5' untranslated end of all viral transcripts. TAR acts as a binding site for viral and cellular proteins. In an attempt to identify RNA ligands that would interfere with the virus life-cycle by interacting with TAR, an in vitro selection was previously carried out. RNA hairpins that formed kissing-loop dimers with TAR were selected [Duconge F. and Toulme JJ (1999) RNA, 5:1605-1614]. We describe here the crystal structure of TAR bound to a high-affinity RNA aptamer. The two hairpins form a kissing complex and interact through six Watson-Crick base pairs. The complex adopts an overall conformation with an inter-helix angle of 28.1 degrees , thus contrasting with previously reported solution and modelling studies. Structural analysis reveals that inter-backbone hydrogen bonds between ribose 2' hydroxyl and phosphate oxygens at the stem-loop junctions can be formed. Thermal denaturation and surface plasmon resonance experiments with chemically modified 2'-O-methyl incorporated into both hairpins at key positions, clearly demonstrate the involvement of this intermolecular network of hydrogen bonds in complex stability. | |||
Exploring TAR-RNA aptamer loop-loop interaction by X-ray crystallography, UV spectroscopy and surface plasmon resonance.,Lebars I, Legrand P, Aime A, Pinaud N, Fribourg S, Di Primo C Nucleic Acids Res. 2008 Dec;36(22):7146-56. Epub 2008 Nov 7. PMID:18996893<ref>PMID:18996893</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Diprimo, C.]] | [[Category: Diprimo, C.]] | ||
[[Category: Fribourg, S.]] | [[Category: Fribourg, S.]] | ||
[[Category: Kissing complex]] | [[Category: Kissing complex]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
Revision as of 15:29, 12 October 2014
CRYSTAL STRUCTURE OF AN RNA KISSING COMPLEX
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