1h21: Difference between revisions

The facultative sulfate/nitrate-reducing bacterium Desulfovibrio, desulfuricans ATCC 27774 harbours a split-Soret cytochrome c. This, cytochrome is a homodimeric protein, having two bis-histidinyl c-type, haems per monomer. It has an unique architecture at the haem domain: each, haem has one of the coordinating histidines provided by the other monomer, and in each monomer the haems are parallel to each other, almost in van, der Waals contact. This work reports the cloning and sequencing of the, gene encoding for this cytochrome and shows, by transcriptional analysis, that it is more expressed in nitrate-grown cells than in sulfate-grown, ones. In addition, the gene-deduced amino acid sequence revealed two new, cysteine residues that could be involved in the binding of a non-haem iron, centre. Indeed, the presence of a novel type of an iron-sulfur centre, (possibly of the [2Fe-2S] type) was demonstrated by EPR spectroscopy, and, putative models for its localization and structure in the cytochrome, molecule are proposed on the basis of the so-far-known 3D crystallographic, structure of the aerobically purified split-Soret cytochrome, which lacks, this centre.

1H21 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_desulfuricans Desulfovibrio desulfuricans] with HEC as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1DDC. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H21 OCA].  

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