1xco: Difference between revisions
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'''Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate''' | {{Structure | ||
|PDB= 1xco |SIZE=350|CAPTION= <scene name='initialview01'>1xco</scene>, resolution 2.85Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=UVW:ACETYLPHOSPHATE'>UVW</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphate_acetyltransferase Phosphate acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.8 2.3.1.8] | |||
|GENE= pta, ipa-88d ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |||
}} | |||
'''Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1XCO is a [ | 1XCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCO OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate., Xu QS, Jancarik J, Lou Y, Kuznetsova K, Yakunin AF, Yokota H, Adams P, Kim R, Kim SH, J Struct Funct Genomics. 2005 Dec;6(4):269-79. Epub 2005 Nov 9. PMID:[http:// | Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate., Xu QS, Jancarik J, Lou Y, Kuznetsova K, Yakunin AF, Yokota H, Adams P, Kim R, Kim SH, J Struct Funct Genomics. 2005 Dec;6(4):269-79. Epub 2005 Nov 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16283428 16283428] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Phosphate acetyltransferase]] | [[Category: Phosphate acetyltransferase]] | ||
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[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:08:04 2008'' | ||
Revision as of 13:08, 20 March 2008
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| 1xco, resolution 2.85Å | |||||||||||||
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| Ligands: | SO4 and UVW | ||||||||||||
| Gene: | pta, ipa-88d (Bacillus subtilis) | ||||||||||||
| Activity: | Phosphate acetyltransferase, with EC number 2.3.1.8 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate
Overview
Phosphotransacetylase (Pta) [EC 2.3.1.8] plays a major role in acetate metabolism by catalyzing the reversible transfer of the acetyl group between coenzyme A (CoA) and orthophosphate: CH(3)COSCoA+HPO(4)(2-)<-->CH(3)COOPO(3)(2-) +CoASH. In this study, we report the crystal structures of Pta from Bacillus subtilis at 2.75 A resolution and its complex with acetyl phosphate, one of its substrates, at 2.85 A resolution. In addition, the Pta activity of the enzyme has been assayed. The enzyme folds into an alpha/beta architecture with two domains separated by a prominent cleft, very similar to two other known Pta structures. The enzyme-acetyl phosphate complex structure reveals a few potential substrate binding sites. Two of them are located in the middle of the interdomain cleft: each one is surrounded by a region of strictly and highly conserved residues. High structural similarities are found with 4-hydroxythreonine-4-phosphate dehydrogenase (PdxA), and isocitrate and isopropylmalate dehydrogenases, all of which utilize NADP+ as their cofactor, which binds in the interdomain cleft. Their substrate binding sites are close to the acetyl phosphate binding sites of Pta in the cleft as well. These results suggest that the CoA is likely to bind to the interdomain cleft of Pta in a similar way as NADP+ binds to the other three enzymes.
About this Structure
1XCO is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate., Xu QS, Jancarik J, Lou Y, Kuznetsova K, Yakunin AF, Yokota H, Adams P, Kim R, Kim SH, J Struct Funct Genomics. 2005 Dec;6(4):269-79. Epub 2005 Nov 9. PMID:16283428
Page seeded by OCA on Thu Mar 20 15:08:04 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Bacillus subtilis
- Phosphate acetyltransferase
- Single protein
- Adams, P.
- BSGC, Berkeley Structural Genomics Center.
- Jancarik, J.
- Kim, R.
- Kim, S H.
- Lou, Y.
- Xu, Q S.
- Yokota, H.
- SO4
- UVW
- Berkeley structural genomics center
- Bsgc
- Bsgc structure funded by nih
- Protein structure initiative
- Psi
- Structural genomic
