1xjy: Difference between revisions
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[[Image:1xjy.gif|left|200px]] | [[Image:1xjy.gif|left|200px]] | ||
'''The crystal structures of the DNA binding sites of the RUNX1 transcription factor''' | {{Structure | ||
|PDB= 1xjy |SIZE=350|CAPTION= <scene name='initialview01'>1xjy</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''The crystal structures of the DNA binding sites of the RUNX1 transcription factor''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1XJY is a [ | 1XJY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJY OCA]. | ||
==Reference== | ==Reference== | ||
Structures of the DNA-binding site of Runt-domain transcription regulators., Kitayner M, Rozenberg H, Rabinovich D, Shakked Z, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):236-46. Epub 2005, Feb 24. PMID:[http:// | Structures of the DNA-binding site of Runt-domain transcription regulators., Kitayner M, Rozenberg H, Rabinovich D, Shakked Z, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):236-46. Epub 2005, Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15735333 15735333] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Kitayner, M.]] | [[Category: Kitayner, M.]] | ||
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[[Category: a-dna]] | [[Category: a-dna]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: overhanging | [[Category: overhanging base]] | ||
[[Category: runx1]] | [[Category: runx1]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:10:47 2008'' | ||
Revision as of 13:10, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
The crystal structures of the DNA binding sites of the RUNX1 transcription factor
Overview
Runt-domain (RD) proteins are transcription factors that play fundamental roles in various developmental pathways. They bind specifically to DNA sequences of the general form PyGPyGGTPy (Py = pyrimidine), through which they regulate transcription of target genes. The DNA duplex TCTGCGGTC/TGACCGCAG, incorporating the binding site for the RD transcription factors (bold), was crystallized in space group P4(3). X-ray analysis of two crystals diffracting to 1.7 and 2.0 angstroms resolution, which had slight variations in their unit-cell parameters, revealed two distinct conformations of the A-DNA helix. The two crystal structures possessed several structure and hydration features that had previously been observed in A-DNA duplexes. A comparative analysis of the present A-DNA structures and those of previously reported B-DNA crystal structures of RD-binding sites in free and protein-bound states showed the various duplexes to display several common features. Within this series, the present A-DNA duplexes adopt two conformations along the pathway from the canonical A-DNA to the B-DNA forms and the protein-bound helices display conformational features that are intermediate between those of the current A-DNA structures and that of the B-DNA-type helix of the free RD target. Based on these data and energy considerations, it is likely that the propensity of the RD-binding site to adopt the A-DNA or B-DNA conformation in solution depends on the sequence context and environmental conditions, and that the transition from either DNA form to the protein-bound conformation involves a small energy barrier.
About this Structure
1XJY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structures of the DNA-binding site of Runt-domain transcription regulators., Kitayner M, Rozenberg H, Rabinovich D, Shakked Z, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):236-46. Epub 2005, Feb 24. PMID:15735333
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