1xk5: Difference between revisions
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'''Crystal structure of the m3G-cap-binding domain of snurportin1 in complex with a m3GpppG-cap dinucleotide''' | {{Structure | ||
|PDB= 1xk5 |SIZE=350|CAPTION= <scene name='initialview01'>1xk5</scene>, resolution 2.4Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=TPG:2,2,7-TRIMETHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE'>TPG</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of the m3G-cap-binding domain of snurportin1 in complex with a m3GpppG-cap dinucleotide''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1XK5 is a [ | 1XK5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XK5 OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1., Strasser A, Dickmanns A, Luhrmann R, Ficner R, EMBO J. 2005 Jul 6;24(13):2235-43. Epub 2005 May 26. PMID:[http:// | Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1., Strasser A, Dickmanns A, Luhrmann R, Ficner R, EMBO J. 2005 Jul 6;24(13):2235-43. Epub 2005 May 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15920472 15920472] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-rna-complex]] | [[Category: protein-rna-complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:10:52 2008'' | ||
Revision as of 13:10, 20 March 2008
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| 1xk5, resolution 2.4Å | |||||||||||||
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| Ligands: | TPG | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of the m3G-cap-binding domain of snurportin1 in complex with a m3GpppG-cap dinucleotide
Overview
In higher eukaryotes the biogenesis of spliceosomal UsnRNPs involves a nucleocytoplasmic shuttling cycle. After the m7G-cap-dependent export of the snRNAs U1, U2, U4 and U5 to the cytoplasm, each of these snRNAs associates with seven Sm proteins. Subsequently, the m7G-cap is hypermethylated to the 2,2,7-trimethylguanosine (m3G)-cap. The import adaptor snurportin1 recognises the m3G-cap and facilitates the nuclear import of the UsnRNPs by binding to importin-beta. Here we report the crystal structure of the m3G-cap-binding domain of snurportin1 with bound m3GpppG at 2.4 A resolution, revealing a structural similarity to the mRNA-guanyly-transferase. Snurportin1 binds both the hypermethylated cap and the first nucleotide of the RNA in a stacked conformation. This binding mode differs significantly from that of the m7G-cap-binding proteins Cap-binding protein 20 (CBP20), eukaryotic initiation factor 4E (eIF4E) and viral protein 39 (VP39). The specificity of the m3G-cap recognition by snurportin1 was evaluated by fluorescence spectroscopy, demonstrating the importance of a highly solvent exposed tryptophan for the discrimination of m7G-capped RNAs. The critical role of this tryptophan and as well of a tryptophan continuing the RNA base stack was confirmed by nuclear import assays and cap-binding activity tests using several snurportin1 mutants.
About this Structure
1XK5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1., Strasser A, Dickmanns A, Luhrmann R, Ficner R, EMBO J. 2005 Jul 6;24(13):2235-43. Epub 2005 May 26. PMID:15920472
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