1y4w: Difference between revisions
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'''Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21''' | {{Structure | ||
|PDB= 1y4w |SIZE=350|CAPTION= <scene name='initialview01'>1y4w</scene>, resolution 1.55Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Fructan_beta-fructosidase Fructan beta-fructosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.80 3.2.1.80] | |||
|GENE= | |||
}} | |||
'''Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1Y4W is a [ | 1Y4W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_awamori Aspergillus awamori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4W OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition., Nagem RA, Rojas AL, Golubev AM, Korneeva OS, Eneyskaya EV, Kulminskaya AA, Neustroev KN, Polikarpov I, J Mol Biol. 2004 Nov 19;344(2):471-80. PMID:[http:// | Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition., Nagem RA, Rojas AL, Golubev AM, Korneeva OS, Eneyskaya EV, Kulminskaya AA, Neustroev KN, Polikarpov I, J Mol Biol. 2004 Nov 19;344(2):471-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15522299 15522299] | ||
[[Category: Aspergillus awamori]] | [[Category: Aspergillus awamori]] | ||
[[Category: Fructan beta-fructosidase]] | [[Category: Fructan beta-fructosidase]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:18:29 2008'' | ||
Revision as of 13:18, 20 March 2008
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| 1y4w, resolution 1.55Å | |||||||||||||
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| Ligands: | NAG and GOL | ||||||||||||
| Activity: | Fructan beta-fructosidase, with EC number 3.2.1.80 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21
Overview
Exo-inulinases hydrolyze terminal, non-reducing 2,1-linked and 2,6-linked beta-d-fructofuranose residues in inulin, levan and sucrose releasing beta-d-fructose. We present the X-ray structure at 1.55A resolution of exo-inulinase from Aspergillus awamori, a member of glycoside hydrolase family 32, solved by single isomorphous replacement with the anomalous scattering method using the heavy-atom sites derived from a quick cryo-soaking technique. The tertiary structure of this enzyme folds into two domains: the N-terminal catalytic domain of an unusual five-bladed beta-propeller fold and the C-terminal domain folded into a beta-sandwich-like structure. Its structural architecture is very similar to that of another member of glycoside hydrolase family 32, invertase (beta-fructosidase) from Thermotoga maritima, determined recently by X-ray crystallography The exo-inulinase is a glycoprotein containing five N-linked oligosaccharides. Two crystal forms obtained under similar crystallization conditions differ by the degree of protein glycosylation. The X-ray structure of the enzyme:fructose complex, at a resolution of 1.87A, reveals two catalytically important residues: Asp41 and Glu241, a nucleophile and a catalytic acid/base, respectively. The distance between the side-chains of these residues is consistent with a double displacement mechanism of reaction. Asp189, which is part of the Arg-Asp-Pro motif, provides hydrogen bonds important for substrate recognition.
About this Structure
1Y4W is a Single protein structure of sequence from Aspergillus awamori. Full crystallographic information is available from OCA.
Reference
Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition., Nagem RA, Rojas AL, Golubev AM, Korneeva OS, Eneyskaya EV, Kulminskaya AA, Neustroev KN, Polikarpov I, J Mol Biol. 2004 Nov 19;344(2):471-80. PMID:15522299
Page seeded by OCA on Thu Mar 20 15:18:29 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Aspergillus awamori
- Fructan beta-fructosidase
- Single protein
- Eneyskaya, E V.
- Golubev, A M.
- Korneeva, O S.
- Kulminskaya, A A.
- Nagem, R A.P.
- Neustroev, K N.
- Polikarpov, I.
- Rojas, A L.
- GOL
- NAG
- Crystallographic structure
- Exo-inulinase
- Glycoside hydrolase family 32
- Native structure
- X-ray structure
