3bwp: Difference between revisions

Line 1:

[[~~Image~~:3bwp.~~png|left|200px~~]]

==Crystal structure of a self-spliced group II intron==

== Structural highlights ==

<table><tr><td colspan='2'>[[3bwp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BWP FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bwp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bwp RCSB], [http://www.ebi.ac.uk/pdbsum/3bwp PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor.

~~{{STRUCTURE_3bwp| PDB=3bwp | SCENE= }}~~

Crystal structure of a self-spliced group II intron.,Toor N, Keating KS, Taylor SD, Pyle AM Science. 2008 Apr 4;320(5872):77-82. PMID:18388288<ref>PMID:18388288</ref>

~~===Crystal structure~~ of ~~a self-spliced group II intron===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_18388288}}~~

== References ==

~~==About this Structure==~~

__TOC__

~~[[3bwp]] is a 1 chain structure. Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3BWP OCA].~~

</StructureSection>

==~~Reference~~==

<~~ref group="xtra">PMID:018388288<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Keating, K S.]]

[[Category: Pyle, A M.]]

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@@ Line 1: / Line 1: @@
-[[Image:3bwp.png|left|200px]]
+==Crystal structure of a self-spliced group II intron==
+<StructureSection load='3bwp' size='340' side='right' caption='[[3bwp]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3bwp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BWP FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bwp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bwp RCSB], [http://www.ebi.ac.uk/pdbsum/3bwp PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Group II introns are self-splicing ribozymes that catalyze their own excision from precursor transcripts and insertion into new genetic locations. Here we report the crystal structure of an intact, self-spliced group II intron from Oceanobacillus iheyensis at 3.1 angstrom resolution. An extensive network of tertiary interactions facilitates the ordered packing of intron subdomains around a ribozyme core that includes catalytic domain V. The bulge of domain V adopts an unusual helical structure that is located adjacent to a major groove triple helix (catalytic triplex). The bulge and catalytic triplex jointly coordinate two divalent metal ions in a configuration that is consistent with a two-metal ion mechanism for catalysis. Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor.
-{{STRUCTURE_3bwp|  PDB=3bwp  |  SCENE=  }}
+Crystal structure of a self-spliced group II intron.,Toor N, Keating KS, Taylor SD, Pyle AM Science. 2008 Apr 4;320(5872):77-82. PMID:18388288<ref>PMID:18388288</ref>
-===Crystal structure of a self-spliced group II intron===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_18388288}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[3bwp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWP OCA].
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:018388288</ref><references group="xtra"/>
 [[Category: Keating, K S.]]
 [[Category: Pyle, A M.]]