3eil: Difference between revisions
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[[ | ==Structure of B-DNA d(CGTTAATTAACG)2 in the presence of Manganese== | ||
<StructureSection load='3eil' size='340' side='right' caption='[[3eil]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3eil]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EIL FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eil RCSB], [http://www.ebi.ac.uk/pdbsum/3eil PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We present the structure of the duplex formed by a fragment of auto-complementary DNA with the sequence d(CGTTAATTAACG). The structure was determined by X-ray crystallography. Up to date it is the first structure presenting the interaction between a DNA oligonucleotide and manganese ions. The presence of Mn2+ creates bonds among the N7 atom of guanines and phosphates. These bonds stabilize and determine the crystallographic network in a P3(2) space group, unusual in oligonucleotide crystals. The crystal structure observed is compared with those found in the presence of Mg2+, Ca2+ and Ni2+, which show different kinds of interactions. The double helices show end-to-end interactions, in a manner that the terminal guanines interact with the minor groove of the neighboring duplex, while the terminal cytosines are disordered. We have chosen this sequence since it contains a TTAA repeat. Such repeats are very rare in all genomes. We suggest that this sequence may be very susceptible to the formation of closely spaced thymine dimers. | |||
The interaction of manganese ions with DNA.,Millonig H, Pous J, Gouyette C, Subirana JA, Campos JL J Inorg Biochem. 2009 Jun;103(6):876-80. Epub 2009 Mar 25. PMID:19375803<ref>PMID:19375803</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Campos, L J]] | |||
== | [[Category: Millonig, H]] | ||
< | [[Category: Pous, J]] | ||
[[Category: Campos, L J | [[Category: Subirana, J A]] | ||
[[Category: Millonig, H | |||
[[Category: Pous, J | |||
[[Category: Subirana, J A | |||
[[Category: B-dna]] | [[Category: B-dna]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Manganese]] | [[Category: Manganese]] | ||
Revision as of 13:22, 19 November 2014
Structure of B-DNA d(CGTTAATTAACG)2 in the presence of Manganese
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