4efq: Difference between revisions
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[[ | ==Bombyx mori lipoprotein 7 - platinum derivative at 1.94 A resolution== | ||
<StructureSection load='4efq' size='340' side='right' caption='[[4efq]], [[Resolution|resolution]] 1.94Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4efq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EFQ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=0AF:7-HYDROXY-L-TRYPTOPHAN'>0AF</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pub|3pub]], [[4efp|4efp]], [[4efr|4efr]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4efq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4efq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4efq RCSB], [http://www.ebi.ac.uk/pdbsum/4efq PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Three crystal structures of a lipoprotein (Bmlp7) of unknown function, a member of the 30 kDa lipoprotein family from mulberry silkworm (Bombyx mori L.) haemolymph, have been determined. The 1.33 A resolution structure is an excellent example of how a precise crystallographic study can contribute to protein identification. The correct sequence of this haemolymph-isolated protein was assigned thanks to superb-quality electron-density maps. Two unexpected cadmium cations were found in this crystal structure [Bmlp7-I(Cd)] and their presence may be connected to a detoxification mechanism in this insect. For a comparison of the metal-binding sites, the crystal structure of a platinum complex (Bmlp7-Pt) was also solved at 1.94 A resolution. The third (2.50 A resolution) structure, of the native protein harvested in a different season (Bmlp7-II), corresponds to a different polymorph with an altered pattern of intermolecular interactions and with a total absence of cadmium ions and highlights the possible involvement of Bmlp7 in the response to environmental pollution. The N-terminal domain of Bmlp7 has a fold resembling a clockwise spiral created by six helices and can be classified as a VHS domain. The C-terminal domain is folded as a beta-trefoil. The biological function of Bmlp7 is unknown, but its structural homology to sugar-binding proteins suggests that, in analogy to other 30 kDa haemolymph lipoproteins, it could play a role as an anti-apoptotic factor or function in the immune response of the insect to fungal infections. | |||
High-resolution structure of Bombyx mori lipoprotein 7: crystallographic determination of the identity of the protein and its potential role in detoxification.,Pietrzyk AJ, Panjikar S, Bujacz A, Mueller-Dieckmann J, Lochynska M, Jaskolski M, Bujacz G Acta Crystallogr D Biol Crystallogr. 2012 Sep;68(Pt 9):1140-51. doi:, 10.1107/S0907444912021555. Epub 2012 Aug 18. PMID:22948915<ref>PMID:22948915</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Bombyx mori]] | [[Category: Bombyx mori]] | ||
[[Category: Bujacz, A | [[Category: Bujacz, A]] | ||
[[Category: Bujacz, G | [[Category: Bujacz, G]] | ||
[[Category: Jaskolski, M | [[Category: Jaskolski, M]] | ||
[[Category: Mueller-Dieckmann, J | [[Category: Mueller-Dieckmann, J]] | ||
[[Category: Panjikar, S | [[Category: Panjikar, S]] | ||
[[Category: Pietrzyk, A J | [[Category: Pietrzyk, A J]] | ||
[[Category: Beta-trefoil]] | [[Category: Beta-trefoil]] | ||
[[Category: Unknown function]] | [[Category: Unknown function]] | ||
[[Category: Vhs domain]] | [[Category: Vhs domain]] | ||
