4awl: Difference between revisions
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[[ | ==The NF-Y transcription factor is structurally and functionally a sequence specific histone== | ||
<StructureSection load='4awl' size='340' side='right' caption='[[4awl]], [[Resolution|resolution]] 3.08Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4awl]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AWL FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1n1j|1n1j]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4awl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4awl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4awl RCSB], [http://www.ebi.ac.uk/pdbsum/4awl PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The sequence-specific transcription factor NF-Y binds the CCAAT box, one of the sequence elements most frequently found in eukaryotic promoters. NF-Y is composed of the NF-YA and NF-YB/NF-YC subunits, the latter two hosting histone-fold domains (HFDs). The crystal structure of NF-Y bound to a 25 bp CCAAT oligonucleotide shows that the HFD dimer binds to the DNA sugar-phosphate backbone, mimicking the nucleosome H2A/H2B-DNA assembly. NF-YA both binds to NF-YB/NF-YC and inserts an alpha helix deeply into the DNA minor groove, providing sequence-specific contacts to the CCAAT box. Structural considerations and mutational data indicate that NF-YB ubiquitination at Lys138 precedes and is equivalent to H2B Lys120 monoubiquitination, important in transcriptional activation. Thus, NF-Y is a sequence-specific transcription factor with nucleosome-like properties of nonspecific DNA binding and helps establish permissive chromatin modifications at CCAAT promoters. Our findings suggest that other HFD-containing proteins may function in similar ways. | |||
Sequence-Specific Transcription Factor NF-Y Displays Histone-like DNA Binding and H2B-like Ubiquitination.,Nardini M, Gnesutta N, Donati G, Gatta R, Forni C, Fossati A, Vonrhein C, Moras D, Romier C, Bolognesi M, Mantovani R Cell. 2013 Jan 17;152(1-2):132-43. doi: 10.1016/j.cell.2012.11.047. PMID:23332751<ref>PMID:23332751</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[NF-Y Transcription Factor|NF-Y Transcription Factor]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Bolognesi, M | [[Category: Bolognesi, M]] | ||
[[Category: Donati, G | [[Category: Donati, G]] | ||
[[Category: Forni, C | [[Category: Forni, C]] | ||
[[Category: Fossati, A | [[Category: Fossati, A]] | ||
[[Category: Gatta, R | [[Category: Gatta, R]] | ||
[[Category: Gnesutta, N | [[Category: Gnesutta, N]] | ||
[[Category: Mantovani, R | [[Category: Mantovani, R]] | ||
[[Category: Moras, D | [[Category: Moras, D]] | ||
[[Category: Nardini, M | [[Category: Nardini, M]] | ||
[[Category: Romier, C | [[Category: Romier, C]] | ||
[[Category: Vonrhein, C | [[Category: Vonrhein, C]] | ||
[[Category: Dna-binding]] | [[Category: Dna-binding]] | ||
[[Category: Nf-y]] | [[Category: Nf-y]] | ||
[[Category: Transcription-dna complex]] | [[Category: Transcription-dna complex]] | ||
Revision as of 15:42, 9 December 2014
The NF-Y transcription factor is structurally and functionally a sequence specific histone
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