4gou: Difference between revisions
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[[ | ==Crystal structure of an RGS-RhoGEF from Entamoeba histolytica== | ||
<StructureSection load='4gou' size='340' side='right' caption='[[4gou]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4gou]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GOU FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fid|4fid]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EHI_010670, EhRGS-RhoGEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 Entamoeba histolytica])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gou OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gou RCSB], [http://www.ebi.ac.uk/pdbsum/4gou PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
G protein signaling pathways, as key components of physiologic responsiveness and timing, are frequent targets for pharmacologic intervention. Here, we identify an effector for heterotrimeric G protein alpha subunit (EhGalpha1) signaling from Entamoeba histolytica, the causative agent of amoebic colitis. EhGalpha1 interacts with this effector and guanosine triphosphatase-accelerating protein, EhRGS-RhoGEF, in a nucleotide state-selective fashion. Coexpression of EhRGS-RhoGEF with constitutively active EhGalpha1 and EhRacC leads to Rac-dependent spreading in Drosophila S2 cells. EhRGS-RhoGEF overexpression in E. histolytica trophozoites leads to reduced migration toward serum and lower cysteine protease activity, as well as reduced attachment to, and killing of, host cells. A 2.3 A crystal structure of the full-length EhRGS-RhoGEF reveals a putative inhibitory helix engaging the Dbl homology domain Rho-binding surface and the pleckstrin homology domain. Mutational analysis of the EhGalpha1/EhRGS-RhoGEF interface confirms a canonical "regulator of G protein signaling" domain rather than a RhoGEF-RGS ("rgRGS") domain, suggesting a convergent evolution toward heterotrimeric and small G protein cross-talk. | |||
Structural Determinants of RGS-RhoGEF Signaling Critical to Entamoeba histolytica Pathogenesis.,Bosch DE, Kimple AJ, Manning AJ, Muller RE, Willard FS, Machius M, Rogers SL, Siderovski DP Structure. 2012 Dec 19. pii: S0969-2126(12)00427-3. doi:, 10.1016/j.str.2012.11.012. PMID:23260656<ref>PMID:23260656</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Entamoeba histolytica]] | [[Category: Entamoeba histolytica]] | ||
[[Category: Bosch, D E | [[Category: Bosch, D E]] | ||
[[Category: Kimple, A J | [[Category: Kimple, A J]] | ||
[[Category: Machius, M | [[Category: Machius, M]] | ||
[[Category: Muller, R E | [[Category: Muller, R E]] | ||
[[Category: Siderovski, D P | [[Category: Siderovski, D P]] | ||
[[Category: Willard, F S | [[Category: Willard, F S]] | ||
[[Category: Dh domain]] | [[Category: Dh domain]] | ||
[[Category: Ehgalpha1]] | [[Category: Ehgalpha1]] | ||
