2kvk: Difference between revisions
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[[Image: | ==Solution structure of ADF/cofilin (LDCOF) from Leishmania donovani== | ||
<StructureSection load='2kvk' size='340' side='right' caption='[[2kvk]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2kvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_donovani Leishmania donovani]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2kd5 2kd5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KVK FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cofilin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5661 Leishmania donovani])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kvk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kvk RCSB], [http://www.ebi.ac.uk/pdbsum/2kvk PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kv/2kvk_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Leishmania donovani ADF/cofilin (LdCof) is a novel member of ADF/cofilin family. LdCof depolymerizes, but does not co-sediment with, rabbit muscle actin filaments. Its F-actin depolymerizing activity is pH independent. Further, it possesses weak F-actin severing activity. In order to better understand its characteristic properties, we have determined the solution NMR structure of LdCof and have analyzed protein backbone dynamics from (15)N-relaxation measurements. The structure of LdCof possesses a conserved ADF/cofilin fold with a central mixed beta-sheet consisting of six beta-strands which is surrounded by five alpha-helices. LdCof structure has conserved G/F-actin binding site which includes the characteristic long kinked alpha-helix (alpha3). LdCof binds to rabbit muscle ADP-G-actin with 1:1 stoichiometry (K(d) approximately 0.2muM). The F-actin binding site is not well formed and analysis of (15)N-relaxation data shows that residues in the beta4-beta5 loop region and C-terminal are relatively flexible, which seems to be a determinant for the low F-actin severing activity of LdCof. | |||
Solution structure and dynamics of ADF/cofilin from Leishmania donovani.,Pathak PP, Pulavarti SV, Jain A, Sahasrabuddhe AA, Gupta CM, Arora A J Struct Biol. 2010 Jul 11. PMID:20627129<ref>PMID:20627129</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Leishmania donovani]] | [[Category: Leishmania donovani]] | ||
[[Category: Arora, A.]] | [[Category: Arora, A.]] | ||
