3vpk: Difference between revisions
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[[ | ==Crystal Structure of 6-Guanidinohexanoyl Trypsin== | ||
<StructureSection load='3vpk' size='340' side='right' caption='[[3vpk]], [[Resolution|resolution]] 1.94Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vpk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VPK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VPK FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=E4B:ETHYL+4-HYDROXYBENZOATE'>E4B</scene>, <scene name='pdbligand=GHS:6-CARBAMIMIDAMIDOHEXANOIC+ACID'>GHS</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vpk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vpk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vpk RCSB], [http://www.ebi.ac.uk/pdbsum/3vpk PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The force driving the conversion from the acyl intermediate to the tetrahedral intermediate in the deacylation reaction of serine proteases remains unclear. The crystal structure of 6-guanidinohexanoyl trypsin was determined at pH 7.0, near the optimum reaction pH, at 1.94 A resolution. In this structure, three water molecules are observed around the catalytic site. One acts as a nucleophile to attack the acyl carbonyl carbon while the other two waters fix the position of the catalytic water through a hydrogen bond. When the acyl carbonyl oxygen oscillates thermally, the water assumes an appropriate angle to catalyze the deacylation. Proteins 2013. (c) 2012 Wiley Periodicals, Inc. | |||
Crystal structure of 6-guanidinohexanoyl trypsin near the optimum pH reveals the acyl-enzyme intermediate to be deacylated.,Masuda Y, Nitanai Y, Mizutani R, Noguchi S Proteins. 2013 Mar;81(3):526-30. doi: 10.1002/prot.24206. Epub 2012 Dec 24. PMID:23161653<ref>PMID:23161653</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[Trypsin|Trypsin]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Trypsin]] | [[Category: Trypsin]] | ||
[[Category: Masuda, Y | [[Category: Masuda, Y]] | ||
[[Category: Mizutani, R | [[Category: Mizutani, R]] | ||
[[Category: Nitanai, Y | [[Category: Nitanai, Y]] | ||
[[Category: Noguchi, S | [[Category: Noguchi, S]] | ||
[[Category: Acyl-enzyme intermediate]] | [[Category: Acyl-enzyme intermediate]] | ||
[[Category: Anti-pancreatitis drug]] | [[Category: Anti-pancreatitis drug]] | ||
| Line 23: | Line 36: | ||
[[Category: Serine protease]] | [[Category: Serine protease]] | ||
[[Category: Serine protease inhibitor]] | [[Category: Serine protease inhibitor]] | ||
Revision as of 14:19, 9 December 2014
Crystal Structure of 6-Guanidinohexanoyl Trypsin
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